Naslov
Emergence of oxacillinases in environmental carbapenem resistant Acinetobacter baumannii associated with clinical isolates

Autori
Goić-Barišić, Ivana ; Hrenović, Jasna ; Kovačić, Ana ; Šeruga Musić, Martina

Izvornik
Microbial drug resistance-mechanisms epidemiology and disease (1076-6294) 22 (2016), 7; 559-563

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Acinetobacter baumannii ; ß-lactamase ; oxacillinase ; multidrug resistance ; rpoB gene ; wastewater

Sažetak
Six carbapenem resistant isolates of Acinetobacter baumannii were recovered from untreated and treated municipal wastewater of the capital City of Zagreb, Croatia. Molecular identification of environmental isolates of A. baumannii was performed by amplification, sequencing and phylogenetic analyses of rpoB gene. The presence of blaOXA genes encoding OXA-type carbapenemases (OXA-51-like, OXA-23, and OXA- 40-like) was confirmed by multiplex PCR and sequencing. Phylogenetic analyses corroborated the affiliation of detected blaOXA genes to three different clusters and showed association of environmental OXAs with those described from clinical isolates. This result suggests that isolates recovered from municipal wastewater are most probably of clinical origin. Furthermore, the presence of OXA-40- like (OXA-72) in an environmental A. baumannii isolate is reported for the first time. Persistence of A. baumannii harbouring the clinically important OXAs in the wastewater treatment process poses a potentially significant source for horizontal gene transfer and implications for wider spread of antibiotic resistance genes.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Kliničke medicinske znanosti, Javno zdravstvo i zdravstvena zaštita

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