Naslov
Murine cytomegalovirus-induced hepatitis is linked to suppression of immune responses

Autori
Štimac, Davor ; Trgovcich, Joanne ; Polić, Bojan ; Pernjak-Pugel, Ester ; Tomac, Jelena ; Jonjić, Stipan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Annual meeting of the Croatian Immunological Society, Periodicum biologorum 99 (1997), suppl. 2 / Vitale, Branko - Zagreb : Hrvatsko prirodoslovno društvo, 1997, P22-P22

Skup
Annual meeting of the Croatian Immunological Society

Mjesto i datum
Zagreb, Hrvatska, 06.11.1997. - 07.11.1997

Vrsta sudjelovanja
Ostalo

Vrsta recenzije
Nije recenziran

Ključne riječi
citomegalovirus; hepatitis
(cytomegalovirus; hepatitis)

Sažetak
Hepatitis is a major clinical complication of human CMV infections. We have investigated the pathogenesis of murine cytomegalovirus-induced hepatitis during lethal and sublethal-infection. While numerous organs and tissues were capable of supporting virus replication, the highest virus titers and most significant morphological changes occurred in liver and spleen. Lethal dose infection resulted in confluent necrosis of the liver and loss of liver function, which correlated with high levels of virus replication in this organ. In mice given a sublethal dose, immune control mechanisms could limit virus replication in the liver by the 5th post-infection day and liver function recovered. Depletion of T cells led severe loss of liver function and death in mice given an otherwise sublethal dose. Virus infection also resulted in a profound, dose-dependent depletion of spleen cell populations. Furthermore, virus infection led to suppression of delayed-type hypersensitivity responses, indicating that virus-induced immunosuppression is an important aspect of MCMV pathogenesis. An adoptive transfer protocol was employed to investigate the selective role of T cell subsets in disease progression. The CD8+ subset of T cells were essential to control of virus replication in liver, recovery of liver function, and resolution of histopathological changes, whereas the CD4+ subset could only partially restore liver function and rescue tissue damage. Taken together, we propose that virus-induced suppression of immune responses play a crucial role in the etiology of MCMV induced hepatitis.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA