Naslov
Dysregulation of microRNA cluster miR-29 in fibrocytes from patients with JAK2V617F-positive primary myelofibrosis

Autori
Božinović, Ksenija ; Manshouri, Taghi ; Estrov, Zeev ; Pilling, Darrell ; Knez, Liza ; Newberry, Kate J ; Bueso-Ramos, Carlos E ; Post, Sean ; Verstovsek, Srdan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Program and Proceedings Leukemia and Lymphoma East and West: Linking Knowledge and Practice / Kantarjian, Hagop ; Labar, Boris ; Nemet, Damir ; Verstovsek, Srdan - : MD Anderson Cancer Center, 2015, 41-41

Skup
Leukemia & Lymphoma East and West: Linking Knowledge and Practice

Mjesto i datum
Dubrovnik, Hrvatska, 23.09.2015. - 27.09.2015

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
microRNA ; MPN ; CD14+ cells

Sažetak
Myeloproliferative neoplasms (MPN) are a class of stem cell–derived hematologic malignancies, characterized by an expansion of one or more myeloid lineages with resulting bone marrow (BM) hypercellularity. A gain-of-function mutation in JAK2, detected in most patients with MPN, induces constitutive activation of JAK2, stimulation of downstream signaling pathways, and activation of several cytokine and growth factor genes. Recent reports have linked tissue fibrosis, such as pulmonary fibrosis, end-stage liver or kidney disease, heart disease and autoimmune disorder to fibrocytes, spindle-shaped fibroblast-like cells that differentiate from a subpopulation of CD14+ monocytes. We therefore investigated changes in the expression of several microRNAs (miR-16, 21, 29a, 29b1, 29b2, 29c, 155, 181a, and 451) which are involved in a variety of biological processes, including cell cycle regulation, differentiation, and development in cultured fibrocytes from patients with primary myelofibrosis. Bone marrow mononuclear cells (MNC) from twenty eight primary myelofibrosis patients and nine normal control bone marrow samples were cultured, and the resulting fibrocytes were used for this study. Using qRT- PCR we found that the levels of miR-16 (P=0.0122), and miR-21 were significantly higher in PMF patients than in normal controls. Expression level of miR-29-b1 and -b2 were lower (P= 0.0007, P= 0.0001) in PMF patients ; however, the expression of miR29-c was higher(P= 0.0028). Dysregulation of miR-29 has been associated with coronary artery fibrosis and we conclude that miR-29 might play a role in bone marrow fibrosis and represent a potential therapeutic target.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA