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Pregled bibliografske jedinice broj: 808750

Nonspecific native elution of proteins and mumps virus in immunoaffinity chromatography


Brgles, Marija; Sviben, Dora; Forčić, Dubravko; Halassy, Beata
Nonspecific native elution of proteins and mumps virus in immunoaffinity chromatography // Journal of chromatography. A, 1447 (2016), 107-114 doi:10.1016/j.chroma.2016.04.022 (međunarodna recenzija, članak, znanstveni)


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Naslov
Nonspecific native elution of proteins and mumps virus in immunoaffinity chromatography

Autori
Brgles, Marija ; Sviben, Dora ; Forčić, Dubravko ; Halassy, Beata

Izvornik
Journal of chromatography. A (0021-9673) 1447 (2016); 107-114

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Immunoaffinity chromatography ; Native elution ; Virus ; Monolith

Sažetak
Immunoaffinity chromatography, based on the antigen-antibody recognition, enables specific purification of any antigen (protein, virus) by its antibody. The problem with immunoaffinity chromatography is the harsh elution conditions required for disrupting strong antigen-antibody interactions, such as low pH buffers, which are often deleterious for the immobilized protein and the protein to be isolated since they can also disrupt the intramolecular forces. Therefore, immunoaffinity chromatography can only be partially used for protein and virus purification. Here we report on a nonspecific elution in immunoaffinity chromatography using native conditions by elution with amino acid solution at physiological pH for which we suppose possible competing mechanism of action. Elution potential of various amino acid solutions was tested using immunoaffinity columns specific for ovalbumin and mumps virus, and protein G affinity column. Results have shown that the most successful elution solutions were those containing imidazole and arginine of high molarity. Imidazole represents aromatic residues readily found at the antigen- antibody interaction surface and arginine is most frequently found on protein surface in general. Therefore, results on their eluting power in immunoaffinity chromatography, which increases with increasing molarity, are in line with the competing mechanism of action. Virus immunoaffinity chromatography resulted in removal on nonviable virus particles, which is important for research and biotechnology purposes. In addition, amino acids are proven stabilizers for proteins and viruses making approach presented in this work a very convenient purification method.

Izvorni jezik
Engleski

Znanstvena područja
Kemija



POVEZANOST RADA


Projekti:
HRZZ-UIP-2013-11-8193 - Kromatografsko pročišćavanje biomolekula i njihova karakterizacija (CHROBIO) (Brgles, Marija, HRZZ - 2013-11) ( CroRIS)

Ustanove:
Sveučilište u Zagrebu

Profili:

Avatar Url Beata Halassy (autor)

Avatar Url Marija Brgles (autor)

Avatar Url Dubravko Forčić (autor)

Poveznice na cjeloviti tekst rada:

doi www.sciencedirect.com

Citiraj ovu publikaciju:

Brgles, Marija; Sviben, Dora; Forčić, Dubravko; Halassy, Beata
Nonspecific native elution of proteins and mumps virus in immunoaffinity chromatography // Journal of chromatography. A, 1447 (2016), 107-114 doi:10.1016/j.chroma.2016.04.022 (međunarodna recenzija, članak, znanstveni)
Brgles, M., Sviben, D., Forčić, D. & Halassy, B. (2016) Nonspecific native elution of proteins and mumps virus in immunoaffinity chromatography. Journal of chromatography. A, 1447, 107-114 doi:10.1016/j.chroma.2016.04.022.
@article{article, author = {Brgles, Marija and Sviben, Dora and For\v{c}i\'{c}, Dubravko and Halassy, Beata}, year = {2016}, pages = {107-114}, DOI = {10.1016/j.chroma.2016.04.022}, keywords = {Immunoaffinity chromatography, Native elution, Virus, Monolith}, journal = {Journal of chromatography. A}, doi = {10.1016/j.chroma.2016.04.022}, volume = {1447}, issn = {0021-9673}, title = {Nonspecific native elution of proteins and mumps virus in immunoaffinity chromatography}, keyword = {Immunoaffinity chromatography, Native elution, Virus, Monolith} }
@article{article, author = {Brgles, Marija and Sviben, Dora and For\v{c}i\'{c}, Dubravko and Halassy, Beata}, year = {2016}, pages = {107-114}, DOI = {10.1016/j.chroma.2016.04.022}, keywords = {Immunoaffinity chromatography, Native elution, Virus, Monolith}, journal = {Journal of chromatography. A}, doi = {10.1016/j.chroma.2016.04.022}, volume = {1447}, issn = {0021-9673}, title = {Nonspecific native elution of proteins and mumps virus in immunoaffinity chromatography}, keyword = {Immunoaffinity chromatography, Native elution, Virus, Monolith} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Citati:





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