Naslov
Eksperimentalna evolucija u crva C. elegans
(Experimental evolution in worm C. elegans)

Autori
Mravak, Antonija

Vrsta, podvrsta i kategorija rada
Ocjenski radovi, diplomski rad, diplomski

Fakultet
Prirodoslovno-matematički fakultet

Mjesto
Split

Datum
04.03

Godina
2016

Stranica
30

Mentor
Puizina, Jasna

Neposredni voditelj
Pellay, Francois Xavier i Simunić, Juraj

Ključne riječi
dugovječnost; otpornost na stres; UV zračenje; toplinski stres
(longevity; stress resistance; UV radiation; heat stress)

Sažetak
In long-lived mutants longevity was shown to be associated with stress resistance. That fact suggests that better coping with environmental challenges plays a role in lifespan regulation. Obtaining stress resistance through an evolutionary process might help to enlighten aging theory. To examine this hypothesis, we performed experimental evolution experiment on Caenorhabditis elegans. Two evolved strains were obtained, one with resistance to heat and other to UV radiation. Worms that were under high temperature as an evolution constraint have shown a significantly better response to heat stress comparing to wild-type. This resistance appeared even several generations after pre-exposure to lower sublethal temperature, so is most unlikely to be the consequence of hormesis. Considering that experimental evolution was done on a relatively small number of generations, lifespan assay showed no difference. For UV evolved worms there was no significant change in both assays, showing that resistance to UV may be harder to acquire. Observation of their state and hazard ratio indicate that irradiation might have caused damage instead of resistance. Both strains might not have reached the significant evolution change that would lead to better stress response and ultimately longevity.

Izvorni jezik
Engleski

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