Naslov
Lactobacillus and Leuconostoc volatilomes in cheese conditions

Autori
Pogačić, Tomislav ; Maillard, Marie-Bernadette ; Leclerc, Aurélie ; Hervé, Christophe ; Chuat, Victoria ; Valence, Florence ; Thierry, Anne

Izvornik
Applied microbiology and biotechnology (0175-7598) 100 (2016), 5; 2335-2346

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Lactobacillus ; Leuconostoc ; Adjunct culture ; Aroma compounds ; Volatilome fingerprinting ; Metabolomics ; Cheese flavour
(Lactobacillus ; Leuconostoc ; Adjunct culture ; Aroma compounds ; Volatilome fingerprinting ; Metabolomics ; Cheese flavou)

Sažetak
New strains are desirable to diversify flavour of fermented dairy products. The objective of this study was to evaluate the potential of Leuconostoc spp. and Lactobacillus spp. in the production of aroma compounds by metabolic fingerprints of volatiles. Eighteen strains, including five Lactobacillus species (Lactobacillus fermentum, Lactobacillus helveticus, Lactobacillus paracasei, Lactobacillus rhamnosus, Lactobacillus sakei) and three Leuconostoc species (Leuconostoc citreum, Leuconostoc lactis, and Leuconostoc mesenteroides) were incubated for 5 weeks in a curd-based slurry medium under conditions mimicking cheese ripening. Populations were enumerated and volatile compounds were analysed by headspace trap gas chromatography– mass spectrometry (GC–MS). A metabolomics approach followed by multivariate statistical analysis was applied for data processing and analysis. In total, 12 alcohols, 10 aldehydes, 7 esters, 11 ketones, 5 acids and 2 sulphur compounds were identified. Very large differences in concentration of volatile compounds between the highest producing strains and the control medium were observed in particular for diacetyl, 2-butanol, ethyl acetate, 3- methylbutanol, 3-methylbutanoic acid and 2- methylbutanoic acid. Some of the characterized strains demonstrated an interesting aromatizing potential to be used as adjunct culture.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Poljoprivreda (agronomija), Biotehnologija

Napomena
Rad je proizašao iz postdoc boravka (ožujak-rujan 2013) u INRA UMR STLO (Rennes, Bretagne, Francuska). Troškove istraživanja je financirala INRA (project FLAIR). Troškovi boravka i putni troškovi su financirani iz vlastitih sredstava (Tomislav Pogačić, znanstveni novak- postdoktorand).

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