Naslov
Comparative karyotype investigations in thewhite-clawed crayfish (Austropotamobius Pallipes Species Complex) and stone crayfish (a. Torrentium) (Decapoda, Astacidae)

Autori
Mlinarec, Jelena ; Porupski, Ines ; Maguire, Ivana ; Klobučar, Goran

Izvornik
Journal of crustacean biology (0278-0372) 36 (2016), 1; 87-93

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
45S rDNA ; Austropotamobius ; Decapoda ; fluorescence in situ hybridization ; karyotype ; sex chromosomes

Sažetak
This study reports for the first time on the chromosome number and karyological characteristics of the endangered European freshwater crayfish genus Austropotamobius (Decapoda, Astacidae). The karyotypes of white-clawed crayfish (Austropotamobius pallipes sp. complex) and stone crayfish (A. torrentium) consisted of 2n = 176 chromosomes arranged into 76 metacentric, 11 metacentric-submetacentric and one heteromorphic acrocentric/metacentric chromosome pair. Fluorochrome staining with 4, 6-diamino-2-phenylindole (DAPI) has revealed distinctive centromeric/pericentromeric as well as interstitial AT-rich blocks in A. pallipes and A. torrentium. Centromeric/pericentromeric AT-rich blocks were observed on all chromosomes, while interstitial AT-rich blocks were observed on some chromosomes, mostly on the larger ones. Fluorescence in situ hybridization (FISH) with the 45S rDNA probe on mitotic metaphases of A. pallipes and A. torrentium have revealed four major Texas-red signals, corresponding to four sites of 45S rDNA. One of the two 45S rDNA bearing chromosome pairs was highly heteromorphic, considering the length of chromosomes as well as the size and intensity of 45S rDNA FISH signal. We assume that the heteromorphic chromosome pair might represents X and Y sex chromosomes suggesting the presence of XX-XY sex determination system in A. pallipes and A. torrentium.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA