Naslov
Effects of Hsp90 inhibition on galectin3 expression in human monocytic cell line THP1

Autori
Dumić, Jerka ; Dabelić, Sanja ; Zorbaz, Tamara ; Šupraha Goreta, Sandra ; Petrik, Jozsef ; Čulić, Ognjen ; Barišić, Karmela

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
The FEBS Journal 282 / - Berlin : Federation of European Biochemical Societies (FEBS), 2015, 156-156

Skup
40th Congress of The Federation of the European Biochemical Societies 'The Biochemical Basis of Life'

Mjesto i datum
Berlin, Njemačka, 04.07.2015. - 09.07.2015

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Hsp90 ; THP-1 ; galektin-3 ; 17-DMAG
(Hsp90 ; THP-1 ; galectin-3 ; 17-DMAG)

Sažetak
Inhibition of Hsp90 is a promising therapeutic approach with clinical relevance for treatment of specific tumour types since both Hsp90 homologous (Hsp90α and Hsp90β) account for maturation and functional stability of a plethora of polypeptides (Hsp90 client proteins), including many proteins involved in tumorigenesis (e.g. antiapoptotic and signaltransduction proteins, transcription factors, Tyrkinase receptors, etc.). Galectin3, a βgalactoside lectin is a multifunctional protein, ubiquitously expressed in both intracellular and extracellular environments as well as on the surface of different cell types. Intracellular galectin3, recognised as a strong antiapoptotic molecule, is well known for its roles being correlated with the development and malignancy of cancers and cancer drug resistance. We studied the effects of antibiotic geldanamycin, 17DMAG [17( dimethylaminoethyl amino)17demethoxygeldanamycin], an orally bioavailable derivative of ansamycin and siRNA directed to Hsp90α mRNA (Hsp90αsiRNA) on the expression of galectin3, different heat shock protein family members and Hsp90 client proteins involved in cell cycle regulation in human monocytic cell line THP1. Although slight transient increase of galectin3 expression was observed after 24 hours of both treatments, with 17DMAG, and with Hsp90αsiRNA, inhibition of Hsp90 had no significant effect on galectin3 expression. 17DMAG slightly induced the expression of both Hsp90α and Hsp90β, while Hsp90αsiRNA treatment resulted in inhibition of Hsp90α expression, but did not affect Hsp90β expression. While 17DMAG tremendously upregulated expression of HSP70, cdk1, p(Tyr)cdk1, and cdk2, and did not affect Hsp27 expression, Hsp90αsiRNA did not affected any of aforementioned proteins. These results encourage for further studies focused on elucidation of galectin3 in apoptosis and tumorigenesis.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

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