Naslov
New perspectives of antitumor therapy - Silencing of heat shock proteins by siRNA nanodelivery

Autori
Barišić, Karmela

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
2. kongres farmaceuta Crne Gore s međunarodnim sudjelovanjem

Mjesto i datum
Bečići, Crna Gora, 28.05.2015. - 31.05.2015

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
RNA interference (RNAi); Heat shock proteins 70 (Hsp70); siRNA; chitosan nanoparticles

Sažetak
RNA interference (RNAi) is a mechanism of posttranscriptional regulation of gene expression. It can be triggered endogenously, by microRNA (miRNA), natural products of RNA polymerase II, or exogenously, by an uptake of an artificial double-stranded RNA, such as shRNA and siRNA. RNAi can be used as a potent therapeutic tool. The aim of this study was to examine a) the possibility of silencing the expression of the heat shock protein 70 (Hsp70), b) the possibility of delivery of siRNA for Hsp70 by chitosan nanoparticles and c) anti-tumor effect of Hsp70 silencing. RNAi was induced in Jurkat and U251N tumor cell lines by small siRNA for Hsp70 which was entrapped in chitosan nanoparticles. Chitosan nanoparticles showed low toxicity, efficient entrapping of Hsp70 siRNA and effective silencing of hsp70 gene in vitro. In Jurkat and U251N tumor cell lines silencing of the Hsp70 caused a decrease in viability of these tumor cell lines in two- and three-dimensional tumor models. RNA interference (RNAi), Heat shock proteins 70 (Hsp70), siRNA, chitosan nanoparticles

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

POVEZANOST RADA