Naslov
Sewage sludge toxicity assessment using earthworm Eisenia fetida: Can biochemical and histopathological analysis provide fast and accurate insight?

Autori
Babić, Sanja ; Barišić, Josip ; Malev, Olga ; Klobučar, Goran ; Topić Popović, Natalija ; Strunjak-Perović, Ivančica ; Krasnići, Nesrete ; Čož-Rakovac, Rozelinda ; Sauerborn Klobučar, Roberta

Izvornik
Environmental science and pollution research (0944-1344) 23 (2016), 12; 12150-12163

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
soil toxicity ; earthworm ; oxidative stress ; lipid peroxidation ; MXR ; histopathology

Sažetak
Sewage sludge (SS) is a complex organic by- product of wastewater treatment plants. Deposition of large amounts of SS can increase the risk of soil contamination. Therefore there is an increasing need for fast and accurate assessment of SS toxic potential. Toxic effects of SS were tested on earthworm Eisenia fetida tissue, at the subcellular and biochemical level. Earthworms were exposed to depot sludge (DS) concentration ratio of 30 % or 70 %, and to undiluted, 100 and 10 times diluted active sludge (AS). The exposure to DS lasted for 24/48 hours (acute exposure), 96 hours (semi- acute exposure) and 7/14/28 days (sub- chronic exposure), and 48 hours for AS. Toxic effects were tested by the measurements of multixenobiotic resistance mechanism (MXR) activity and lipid peroxidation levels, as well as the observation of morphological alterations and behavioural changes. Biochemical markers confirmed the presence of MXR inhibitors in the tested AS and DS and highlighted the presence of SS-induced oxidative stress. The MXR- inhibition and thiobarbituric acid reactive substances (TBARS) concentration in the whole earthworm's body were higher after the exposition to lower concentration of the DS. Furthermore, histopathological changes revealed damage to earthworm body wall tissue layers as well as to the epithelial and chloragogen cells in the typhlosole region. These changes were proportional to SS concentration in tested soils and to exposure duration. Obtained results may contribute to the understanding of SS- induced toxic effects on terrestrial invertebrates exposed through soil contact and to identify defence mechanisms of earthworms.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA