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Pregled bibliografske jedinice broj: 790492

Electromagnetic fields at the mobile phone frequency (900 MHz) trigger the onset of the general stress response along with DNA modifications in Eisenia fetida earthworms

Bourdineaud, J.P.; Šrut Maja; Tkalec, Mirta; Brethes, D.; Malarić, Krešimir; Klobučar Goran
Electromagnetic fields at the mobile phone frequency (900 MHz) trigger the onset of the general stress response along with DNA modifications in Eisenia fetida earthworms // 12th Croatian Biological Congress with International Participation / Klobučar, Goran ; Kopjar, Nevenka ; Udovič, Marija GLigora ; Lukša, Žaklin ; Jelić, Dušan (ur.).
Zagreb: Hrvatsko biološko društvo, 2015. str. 122-122 (predavanje, međunarodna recenzija, sažetak, ostalo)

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Naslov
Electromagnetic fields at the mobile phone frequency (900 MHz) trigger the onset of the general stress response along with DNA modifications in Eisenia fetida earthworms

Autori
Bourdineaud, J.P. ; Šrut Maja ; Tkalec, Mirta ; Brethes, D. ; Malarić, Krešimir ; Klobučar Goran

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
12th Croatian Biological Congress with International Participation / Klobučar, Goran ; Kopjar, Nevenka ; Udovič, Marija GLigora ; Lukša, Žaklin ; Jelić, Dušan - Zagreb : Hrvatsko biološko društvo, 2015, 122-122

Skup
12th Croatian Biological Congress

Mjesto i datum
Sveti Martin na Muri, Hrvatska, 18.09.2015. - 23.09.2015

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
DNA modifications; earthworm; exposure to electromagnetic field; stress; Western blotting

Sažetak
Eisenia fetida earthworms were exposed to electromagnetic field (EMF) at the mobile phone frequency (900 MHz), and at field levels ranging from 10 to 120 V m‐1 for a period of 2 h. Potential effects of longer exposure (4 h), field modulation, and recovery period of 24 h after 2 h of exposure were addressed at the field level of 23 V m‐1 . All exposure treatments induced significant DNA modifications as assessed by a quantitative random amplified polymorphic DNA‐ PCR. Even after 24 h of recovery following a 2 h exposure, the number of probe hybridization sites displayed a significant 2‐fold decrease as compared to untreated control earthworms, implying a loss of hybridization sites. Expression of genes involved in the response to general stress (HSP70 and MEKK1, the latter involved in signal transduction), oxidative stress (CAT, encoding catalase), chemical and immune defence (LYS, encoding lysenin, and MYD, encoding a myeloid differentiation factor) were up regulated after exposure to 10 and modulated 23 V m‐1 field levels. Western blots showing an increased quantity of HSP70 and MTCO1 proteins confirmed this stress response (MTCO1 is the subunit 1 of cytochrome c oxidase, indicating a mitochondrial impact). HSP70 and LYS genes were found up regulated after 24 h of recovery following a 2 h exposure, meaning that the effect of EMF exposure lasted for hours.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Elektrotehnika



POVEZANOST RADA

Ustanove:
Fakultet elektrotehnike i računarstva, Zagreb,
Prirodoslovno-matematički fakultet, Zagreb

Profili:

Avatar Url Goran Klobučar (autor)

Avatar Url Krešimir Malarić (autor)

Avatar Url Mirta Tkalec (autor)

Avatar Url Maja Šrut (autor)

www.hbd-sbc.hr

Citiraj ovu publikaciju:

Bourdineaud, J.P.; Šrut Maja; Tkalec, Mirta; Brethes, D.; Malarić, Krešimir; Klobučar Goran
Electromagnetic fields at the mobile phone frequency (900 MHz) trigger the onset of the general stress response along with DNA modifications in Eisenia fetida earthworms // 12th Croatian Biological Congress with International Participation / Klobučar, Goran ; Kopjar, Nevenka ; Udovič, Marija GLigora ; Lukša, Žaklin ; Jelić, Dušan (ur.).
Zagreb: Hrvatsko biološko društvo, 2015. str. 122-122 (predavanje, međunarodna recenzija, sažetak, ostalo)
Bourdineaud, J., Šrut Maja, Tkalec, M., Brethes, D., Malarić, K. & Klobučar Goran (2015) Electromagnetic fields at the mobile phone frequency (900 MHz) trigger the onset of the general stress response along with DNA modifications in Eisenia fetida earthworms. U: Klobučar, G., Kopjar, N., Udovič, M., Lukša, Ž. & Jelić, D. (ur.)12th Croatian Biological Congress with International Participation.
@article{article, author = {Bourdineaud, J.P. and Tkalec, Mirta and Brethes, D. and Malari\'{c}, Kre\v{s}imir}, year = {2015}, pages = {122-122}, keywords = {DNA modifications, earthworm, exposure to electromagnetic field, stress, Western blotting}, title = {Electromagnetic fields at the mobile phone frequency (900 MHz) trigger the onset of the general stress response along with DNA modifications in Eisenia fetida earthworms}, keyword = {DNA modifications, earthworm, exposure to electromagnetic field, stress, Western blotting}, publisher = {Hrvatsko biolo\v{s}ko dru\v{s}tvo}, publisherplace = {Sveti Martin na Muri, Hrvatska} }
@article{article, author = {Bourdineaud, J.P. and Tkalec, Mirta and Brethes, D. and Malari\'{c}, Kre\v{s}imir}, year = {2015}, pages = {122-122}, keywords = {DNA modifications, earthworm, exposure to electromagnetic field, stress, Western blotting}, title = {Electromagnetic fields at the mobile phone frequency (900 MHz) trigger the onset of the general stress response along with DNA modifications in Eisenia fetida earthworms}, keyword = {DNA modifications, earthworm, exposure to electromagnetic field, stress, Western blotting}, publisher = {Hrvatsko biolo\v{s}ko dru\v{s}tvo}, publisherplace = {Sveti Martin na Muri, Hrvatska} }

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