Naslov
Influence of magnesium ions on the structure of DNA investigated by FTIR spectroscopy

Autori
Serec, Kristina ; Dolanski Babic, Sanja ; Tomic, Silvia

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
10th Christmas Biophysics Workshop, Book of Abstracts / D’Adamo, Giuseppe ; Micheletti, Cristian - Trst, 2015, 29-29

Skup
10th Christmas Biophysics Workshop

Mjesto i datum
San Daniele del Friuli, Italija, 14-15.12.2105

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Nije recenziran

Ključne riječi
vibrational spectroscopy; divalent cations; DNA structure

Sažetak
Previous research showed that under influence of Mg+2 cations DNA undergoes structural and conformational changes [1, 2, 3, 4, 5, 6]. It was pointed out by many authors that regardless of the conditions, Mg ions show great affinity toward phosphate groups of DNA, thus neutralizing and stabilizing double helical structure. Interaction of the Mg ions with the base sites, however, still remains unclear as different authors report different affinities and preferable sites of Mg-base coordination [1, 2, 5, 6]. Thus, possibility and particular mechanism of Mg-DNA interaction still remains unclear, as experimental data is often not consistent. In order of finding the best model, we developed protocols where Mg-DNA interaction can be tested with and without intrinsic Na cations, and over large interval of molar concentrations ratio (r=[Mg2+]/[P]). Fourier Transform Infrared spectroscopy was used to determine the effects of Mg+2 ions on the structure and conformation of DNA in thin films. Since DNA is highly sensitive to quantity of metal ions, samples were prepared in such a way as to have r over 4 orders of magnitude (r= 0.0067 to 30). Also, in order to eliminate/determine influence of sodium ions extensive dialysis was performed, and then compared with samples containing sodium ions. References [1] Bhattacharyya, R.G., K.K. Nayak, and A.N. Chakrabarty, Inorganica Chimica Acta-Bioinorganic Chemistry, 1988. 153(2): p. 79-86. [2] Langlais, M., H.A. Tajmirriahi, and R. Savoie, Biopolymers, 1990. 30(7-8): p. 743-752. [3] Duguid, J., et al., Biophysical Journal, 1993. 65(5): p. 1916-1928. [4] Duguid, J.G., et al., Biophysical Journal, 1995. 69(6): p. 2623-2641. [5] Hackl, E.V., S.V. Kornilova, and Y.P. Blagoi, International Journal of Biological Macromolecules, 2005. 35(3-4): p. 175-191. [6] Ahmad, R., H. Arakawa, and H.A. Tajmir-Riahi, Biophysical Journal, 2003. 84(4): p. 2460-2466. 29

Izvorni jezik
Engleski

Znanstvena područja
Fizika

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