Naslov
Cytokine expression in synovial membrane of sheep knee treated with tissue engineered osteochondral graft

Autori
Pušić, Maja ; Vukasović, Andreja ; Kostešić, Petar ; Matičić, Dražen ; Hudetz, Damir ; Ježek, Davor ; Pećina, Marko ; Ivković, Alan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
SEEFORT - Book of Abstracts / - , 2015

Skup
Southeast Europe Forum on Orthopaedics and Traumatology

Mjesto i datum
Dubrovnik, Hrvatska, 23.04.2015. - 25.04.2015

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
sheep ; synovial membrane ; IL-6 ; IL-1beta ; TNFalpha

Sažetak
The aim of this study was to apply bioreactor engineered tissue graft for the treatment of osteochondral defects in sheep, thus enabling cartilage restoration, and examine tissue reactivity to this type of treatment. 8 sheep were assigned to 4 treatment groups. All sheep underwent two surgical procedures. During the first procedure, chondral defects 4 mm in diameter were created on the weight bearing surfaces of both condyles of the right femur. After six weeks, all chondral defects were converted to osteochondral defects 6 mm in diameter and 5 mm deep. In groups NC (n=2) and AC (n=2) autologous three-dimensional osteochondral grafts engineered in perfusion bioreactor from scaffolds with nasal septum and articular chondrocytes respectively were implanted in the defect. In the CFS group (n=2) only scaffolds were implanted while CTR group (n=2) underwent defect conversion only (negative control). 6 weeks after treatment surgery, all sheep were sacrificed and tissue blocks were obtained for histology analysis. The samples were fixed in 4% buffered paraformaldehyde and decalcified in 15% EDTA. Paraffin blocks were sectioned at 5 µm and stained with hematoxylin-eosin and against TNF- alpha. The amount of TNF-alpha, IL-1β and IL-6 mRNA expressed in synovial membrane of treated and contralateral knees was measured with qRT- PCR. Results showed moderate expression of all three cytokines with no significant difference among groups. The research leading to these results has received funding from the European Union's Seventh Framework Programme (FP7/2007- 2013) under grant agreement n°278807.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Veterinarska medicina

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