Naslov
Bioreactor-engineered cartilage graft for osteochondral knee lesion – study in sheep

Autori
Vukasović, Andreja ; Kostešić, Petar ; Pušić, Maja ; Matičić, Dražen ; Hudetz, Damir ; Ježek, Davor ; Pećina, Marko ; Ivković, Alan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
SEEFORT - Book of abstracts / - , 2015, 119-119

Skup
Southeast Europe Forum on Orthopaedics and Tramatology

Mjesto i datum
Dubrovnik, Hrvatska, 23.04.2015. - 25.04.2015

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
bioreactor ; tissue engineering ; cartilage ; knee

Sažetak
The aim of this study was to apply bioreactor engineered tissue graft for the treatment of osteochondral defects in sheep stifle joint, thus enabling cartilage and subchondral bone restoration. 8 sheep were assigned to 4 treatment groups. All sheep underwent two surgical procedures. During the first procedure, chondral defects 4 mm in diameter were created on the weight bearing surfaces of both condyles of the right femur. After six weeks, all chondral defects were converted to osteochondral defects 6 mm in diameter and 5 mm deep. In groups NC (n=2) and AC (n=2) autologous three-dimensional osteochondral grafts engineered in perfusion bioreactor from bilayered scaffolds and nasal septum chondrocytes or articular chondrocytes respectively were implanted in the defect. In the CFS group (n=2) only bilayered scaffolds were implanted while CTR group (n=2) underwent defect conversion only (negative control). Three months after treatment surgery, all sheep were sacrificed and tissue blocks were obtained for histology analysis. The samples were fixed in 4% buffered paraformaldehyde and decalcified in 15% EDTA. Paraffin blocks were sectioned at 5 µm and stained with hematoxylin-eosin, safranin O and picrosirius Red. Immunohistochemistry was performed against collagen type I, II and aggrecan. Histology was evaluated with ICRS II histological score. Overall results showed that cartilage restoration was the best in NC group. Matrix staining, ICRS II parameter, scored superiorly in NC group (p<0.05). The research leading to these results has received funding from the European Union's Seventh Framework Programme (FP7/2007-2013) under grant agreement n°278807.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti, Veterinarska medicina

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