Naslov
Determination of class III peroxidase and laccase isoforms during development of barley (Hordeum vulgare L.) stem

Autori
Begović, Lidija ; Pavoković, Dubravko ; Mlinarić, Selma ; Cesar, Vera

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Zbornik sažetaka 12. Hrvatskog biološkog kongresa s međunarodnim sudjelovanjem / Klobučar, Göran ; Kopjar, Nevenka ; Gligora Udovič, Marija ; Lukša, Žaklin ; Jelić, Dušan - Zagreb : Hrvatsko biološko društvo, 2015, 100-100

Skup
12. HRVATSKI BIOLOŠKI KONGRES s međunarodnim sudjelovanjem

Mjesto i datum
Sveti Martin na Muri, Hrvatska, 18.09.2015. - 23.09.2015

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
internode; cell wall; lignin; isoformes; multicopper oxidase

Sažetak
Class III peroxidases are involved in many physiological processes in plants. Together with laccases they are involved in oxidation of monolignols as the final step in the synthesis of lignin. Lignin is a natural polymer that builds the cell wall and provides mechanical strength to the stem. In the present study, objective was to determine the characteristics of individual isoforms of peroxidase and laccase in internodes of barley during the four developmental stages: elongation, heading, anthesis and grain filling. Total peroxidase (tPOD), laccase and peroxidase covalently bound to the cell wall (cPOD) were extracted and separated using isolelectric focusing on polyacrylamide gels. Guaiacol was used for detection of peroxidase activity and syringaldazine for laccase activity. The analysis showed the presence of eight different isoforms of tPOD with isoelectric point (pI) 8-10 and one isoform with pI 2.5-5. Highest activity was recorded in the first internode in all developmental stages. cPOD were present in a single isoform with activity profile similar to tPOD. Laccase were represented with two isoforms (pI 8-10 and pI 2.5-5) with no difference in activity in a particular internode and developmental stage. The results show the presence and characteristics of the various isoforms of peroxidase and laccase involved in development of barley stem. Future studies aim to clarify the role of these enzymes in the biosynthesis of lignin.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA