Naslov
Protective effects of resveratrol in ochratoxin A and citrinin-induced oxidative stress

Autori
Peraica, Maja ; Rašić, Dubravka ; Mladinić, Marin ; Želježić, Davor

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
51st Congress of the European Societies of Toxicology (EUROTOX) : abstracts ; u: Toxicology Letters 238 (2015) (S2) ; S1-S384 ; Poster Session ; P13-Mechanisms of Toxicity / - , 2015, S296-S296

Skup
Congress of the European Societies of Toxicology (61 ; 2015))

Mjesto i datum
Porto, Portugal, 13.09.2015. - 16.09.2015

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Resveratrol; ochratoxin A; citrinin; oxidative stress; kidney; liver

Sažetak
Our previous studies have found that the nephrotoxic mycotoxin ochratoxin A (OTA) causes oxidative stress in rats and that this effect increases in combination with citrinin (CTN). Resveratrol (RSV) is a well-known antioxidative compound found in wine, grapes, dark chocolate and various berries. The purpose of this study was to establish whether RSV treatment could counteract oxidative stress in animals treated with OTA and CTN. Animals were treated orally in groups of 6 with OTA (0.125 and 0.250 mg kg−1 b.w.) with or without RSV (20 mg kg −1 b.w.) for 21 days. All of the animals were given CTN (20 mg kg−1 b.w.) orally on the last 2 days of OTA treatment. In the kidney and liver, the target organs of OTA and CTN toxicity, of both the treated animals and controls, glutathione (GSH) and malondialdehyde (MDA) concentrations were measured using spectrophotometry and HPLC, respectively. Oxidative DNA damage in kidney and liver homogenates was measured using the hOGG1 modified comet assay. RSV had a significant protective effect against oxidative stress caused by both doses of OTA (0.125 and 0.250 mg kg−1 b.w.) and CTN (20 mg kg −1 b.w.). The GSH concentration was higher in the kidney (0.304 ± 0.045, 0.256 ± 0.046 g g−1 tissue) of animals given both doses of OTA and CTN + RSV than that of animals given both OTA doses and CTN (0.200 ± 0.056, 0.164 ± 0.025 g g−1 tissue, p < 0.05). The same effect of RSV was seen in the liver regarding GSH (0.449 ± 0.081, 0.500 ± 0.100 g g−1 tissue, p < 0.05) compared to animals treated with OTA + CTN (0.300 ± 0.022, 0.298 ± 0.021 g g−1 tissue). RSV reduced MDA concentration in kidney of animals treated with OTA (0.250 mg kg−1 b.w.) + CTN + RSV (28.27 ± 3.33 ng g−1 tissue) compared to OTA + CTN treatment (42.48 ± 2.92) and in the liver of animals treated with OTA (0.125 mg kg−1) + CTN + RSV (11.1 ± 1.15) compared to controls (27.25 ± 2.90, p < 0.05). The tail intensity in the hOGG1 comet assay revealed RSV protection in the liver of animals treated with a lower dose of OTA + CTN, while DNA damage at the higher OTA dose + CTN remained significant (p < 0.05). RSV did not protect the kidney tissue from DNA oxidative damage from both OTA doses and CTN.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

Napomena
DOI: 10.1016/j.toxlet.2015.08.849

POVEZANOST RADA