Pregled bibliografske jedinice broj: 788533
VERIFICATION OF QUANTITATIVE REAL-TIME (qPCR) microRNA PROFILING
VERIFICATION OF QUANTITATIVE REAL-TIME (qPCR) microRNA PROFILING // Clinical Chemistry and Laboratory Medicine
Zagreb, Hrvatska: Walter de Gruyter, 2015. str. eA111-eA148 (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 788533 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
VERIFICATION OF QUANTITATIVE REAL-TIME (qPCR) microRNA PROFILING
Autori
Kardum Paro, Mirjana Mariana ; Flegar- Meštrić, Zlata ; Hoareau- Aveilla Coralie ; Quelen Cathy ; Bousquet Marina ; Brousset, Pierre
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Clinical Chemistry and Laboratory Medicine
/ - : Walter de Gruyter, 2015, EA111-eA148
Skup
15th EFLM Continuous Postgraduate Course in Clinical Chemistry and Laboratory Medicine. How to assess the quality of your method?
Mjesto i datum
Zagreb, Hrvatska, 24.10.2015. - 25.10.2015
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
microRNA; serum; plasma; MIQE; qPCR
Sažetak
MicroRNAs (miRNAs) derived from various tissues or organs have critical functions in many biological processes. miRNA profiling either in human serum or plasma could be clinically usefull in noninvasive molecular diagnostic testing, but its clinical effectiveness is likely to be affected by various methodological issues like the efficiency of RNA recovery and qPCR platform used. The aim of this study was verification of miRNAs profiling performance on TaqMan qPCR platform. Verification of qPCR miRNA profiling on TaqMan qPCR platform according to MIQE guidelines and manufacturers recommendations demonstrated good reproducibility which is a prerequisite for its clinical application.
Izvorni jezik
Engleski
Znanstvena područja
Kliničke medicinske znanosti
POVEZANOST RADA
Ustanove:
Klinička bolnica "Merkur"
