Naslov
CFEX (Slc26a6) in rat kidneys, liver, and small intestine in an experimental model of oxalate nephrolitiasis

Autori
Karaica, Dean ; Breljak, Davorka ; Brzica, Hrvoje ; Lončar, Jovica ; Ljubojević, Marija ; Herak-Kramberger, Carol M. ; Micek, Vedran ; Vrhovac, Ivana ; Ivković Dupor, Jana ; Mihaljević, Ivan ; Marić, Petra ; Smital, Tvrtko ; Burckhardt, Birgitta C. ; Burckhardt Gerhard ; Sabolić, Ivan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Croatian Symposium on Membrane Transporters: abstracts, Archives of Industrial Hygiene and Toxicology vol. 66 sv. 3 / Kopjar, Nevenka - Zagreb : Institut za medicinska istraživanja i medicinu rada, 2015, 228-228

Skup
The 2nd Croatian Symposium on Membrane Transporters

Mjesto i datum
Zagreb, Hrvatska, 27.10.2015

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
ethylene glycol ; hyperoxaluria ; immunocytochemistry ; membrane transporters ; proximal tubule ; qRT-PCR ; urolithiasis ; Western blotting

Sažetak
CFEX (chloride/formate exchanger ; Slc26a6) is an important anion exchanger of chloride, bicarbonate, oxalate (OX), formate, and hydroxyl ions in kidneys, liver, and the small intestine. Studies on CFEX-knockout mice indicated a possible role of CFEX in the development of hyperoxaluria and OX urolithiasis/nephrolithiasis, which in humans is more frequent in men. Here we studied the expression of the CFEX protein and mRNA in the organs of male and female rats, and employed a rat model of ethylene glycol (EG)-induced OX urolithiasis in order to correlate the expression of CFEX with sex-related hyperoxaluria. Rats drank EG in water (0.75 % vol/vol) or water (control) for 30 days. Tissue expressions of the CFEX protein and mRNA were analysed by immunochemical methods and qRT-PCR, respectively. The specificity of an anti-CFEX antibody, used in immunochemical studies, was confirmed in HEK293 cells transiently transfected with CFEX cDNA. In kidneys, the CFEX protein was immunolocalized to the proximal tubule brush-border membrane (BBM) with segmental (S3>>S1~S2) and sex (male>female) differences. Sex-unrelated expression was detected in the BBM of enterocytes (duodenum>jejunum) and in the hepatocyte canalicular membrane. In immunoblots, the CFEX protein band of ~120 kDa in various organs showed an expression pattern comparable to that in immunocytochemistry ; however, renal CFEX mRNA expression was not sex-dependent. Compared to controls and EG-treated females, the EG-treated male rats exhibited hyperoxalemia, hyperoxaluria and OX crystaluria, but the expression of CFEX mRNA and protein remained unaffected in the organs of both sexes. Thus, basic CFEX expression in both rat sexes was sufficient for OX handling even upon EG-treatment, indicating that in rats, CFEX plays no major role in generating EG-induced hyperoxaluria and nephrolithiasis.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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