Naslov
Genotipizacija izolata Brucella melitensis u Republici Hrvatskoj metodom MLVA
(MLVA typing of isolates of Brucella melitensis isolated in the Republic of Croatia)

Autori
Cvetnić, Željko ; Zdelar-Tuk, Maja ; Duvnjak, Sanja ; Račić, Ivana ; Škrivanko, Mario ; Špičić, Silvio

Izvornik
Veterinarski arhiv. Supplement (0372-5480) 85 (2015), 5; 481-492

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
MLVA; B. melitensis; preživači; Hrvatska
(MLVA; B. melitensis; ruminants; Croatia)

Sažetak
In the period from 2009 to 2013, bacteriological testing was conducted on 336 sheep, goat and cattle samples. Using classical bacteriological and molecular procedures, B. melitensis was confirmed in 14 (4.2 %) samples. Brucella was isolated in Karlovac, Lika-Senj and Split-Dalmatia Counties. Brucella isolates were genotyped using the MLVA method and compared to isolates from neighbouring Bosnia and Herzegovina (BH). A total of 14 isolates (strains) originating from Croatia and 25 from BH were analysed. Complete matches between Croatian and BH isolates were found in two genotype groups. Overall, the MLVA analysis indicated that Croatian and BH B. melitensis genotypes from animals were highly homogenous. The Hunter Gaston diversity index (HGDI) higher than 0 was found only for 5 panel 2B loci (Bruce 04, 07, 09, 16, 30). The discriminating loci are optimal for use in rapid epidemiological investigation of B. melitensis infections in these two countries.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA