Naslov
Assessment of primary DNA damage in organs of Wistar rats after acute exposure to different silver nanoparticles

Autori
Milić, Mirta ; Ljubojević, Marija ; Ćurlin, Marija ; Micek, Vedran ; Jurašin, Darija ; Pavičić, Ivan ; Gajović, Srećko ; Vinković Vrček, Ivana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
XIth ISTERH Conference Recent advances in trace element research in health and disease : abstracts ; u: Journal of Trace Elements in Medicine and Biology, Special Section / Jurasović, Jasna ; Aschner, Michael - Amsterdam : Elsevier, 2015, 26-26

Skup
ISTERH Conference Recent advances in trace element research in health and disease (11 ; 2015)

Mjesto i datum
Srebreno, Hrvatska, 18.10.2015. - 22.10.2015

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
silver nanoparticles ; genotoxicity ; Wistra rat ; Comet assay

Sažetak
Silver nanoparticles (AgNPs), due to their biocidal activity, are commonly used as a coating or embedding agents for wound dressings, surgical instruments, implants and bone prostheses, odor-resistant textiles, deodorants, room sprays, water cleaners, laundry detergents, in food packaging and wall paints. The increasing use of nanosilver-containing products poses toxicological and environmental issues which need to be addressed. Here, we present results on primary DNA damage in blood, kidney and liver of 3 months old male and female Wistar rats after 30 min exposure to 1 mg/kg b.w. of silver nanoparticles (AgNPs). Comparison was done for effects of 3 types of AgNPs coated with polyvinylpyrrolidone (PVPAgNPs), metallothionein (METAgNPs), and bovine serum albumin (BSAAgNPs). Tail length, tail intensity (TI) and tail moment were measured by the alkaline comet assay. All experiments were performed in triplicate. In total, 300 comets per concentration were measured. Statistical analysis was performed for TI as recommended by OECD for in vivo genotoxicity testing of chemicals. In the female group, significantly higher DNA damage compared to controls was seen in blood and liver for all AgNPs types, while in kidney samples only BSAAgNPs treatment induced significantly higher TI. In male group, only liver showed significantly higher TI than controls for all three types of AgNPs. In male blood, only METAgNPs induced significant DNA damage compared to controls, while kidneys of males treated with PVPAgNPs and BSAAgNPs showed significantly lower TI values than controls. Since the physicochemical properties of AgNPs such as particle size, surface charge, surface functionalization, and dispersibility significantly influence the degree and actions of AgNPs, further research is required to assess the effects of these variables on their toxicity.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Javno zdravstvo i zdravstvena zaštita

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