Naslov
Biocompatibility of maghemite nanoparticles with different surface coatings

Autori
Pongrac, Igor ; Pavičić, Ivan ; Milić, Mirta ; Brkić, Lada ; Babič, Michal ; Horak, Daniel ; Vinković Vrček, Ivana ; Gajović, Srećko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
XIth ISTERH Conference Recent advances in trace element research in health and disease : abstracts ; u: Journal of Trace Elements in Medicine and Biology, Special Section / Jurasović, Jasna ; Aschner, Michael - Amsterdam : Elsevier, 2015, 30-30

Skup
ISTERH Conference Recent advances in trace element research in health and disease (11 ; 2015)

Mjesto i datum
Srebreno, Hrvatska, 18.10.2015. - 22.10.2015

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
superparamagentic iron oxide nanoparticles ; biocompatibility ; neural stem cells

Sažetak
The biomedical application of maghemite nanoparticles (-Fe2O3NPs) is intended for magnetic resonance and multimodal imaging, thermo-therapy, drug delivery, gene therapy. This study aimed to evaluate the biocompatibility of different -Fe2O3NPs by in vitro testing of their effects on oxidative stress response in neural stem cells (NSC) using staining methods with DCFH-DA, DHE and monochlorobimane molecular probes. The effect of uncoated (UNγ-Fe203NPs), poly-L-lysine-coated (PLLγ-Fe203NPs) and D-manose-coated (MANγ-Fe203NPs) maghemite NPs on intracellular reactive oxygen species (ROS) formation in NSC was evaluated. Significant decrease in ROS levels was obtained in NSC after treatment with MANγ-Fe203NPs and PLLγ-Fe203NPs as measured by the DHE assay, while significant decrease in ROS production was observed for UNγ-Fe203NPs from DCFH-DA staining. Exposure of NSC to all 3 types of γ-Fe203NPs resulted in dose-dependent reduction in the intracellular GSH levels. This indicates that all 3 types of γ-Fe203NPs initiated oxidative stress response in NSC. (Supported by EU FP7 grant GlowBrain: REGPOT-2012-CT2012-316120.)

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Temeljne medicinske znanosti

Napomena
The study was supported by EU FP7 grant GlowBrain (REGPOT-2012-CT2012-316120).

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