Naslov
Biocompatibility of silver nanoparticles as function of surface functionalization: a neural stem cell model

Autori
Pavičić, Ivan ; Milić, Mirta ; Pongrac, Igor ; Ćurlin, Marija ; Jurašin, Darija ; Vinković Vrček, Ivana ; Gajović, Srećko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Journal of Trace Elements in Medicine and Biology / Jurasović, Jasna ; Aschner, Michael - München : Elsevier, 2015, 29-29

Skup
Abstracts XI ISTERH Conference, Recent Advances in Trace Element Reseacrh in Health and Disease

Mjesto i datum
Dubrovnik, Hrvatska, 18.10.2015. - 22.10.2015

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
silver nanoparticles; biocompatibility; surface coating; neural stem cells

Sažetak
Silver nanoparticles (AgNPs) are the most commonly used nanomaterials with focus on their biocidal activity commonly used for medical purposes. This study aimed to understand in vitro nanotoxicity by evaluating the effect of 5 different AgNPs (stabilized with different surface coating: sodium bis(2-ethylhexyl)-sulfosuccinate (AOTAgNP), cetyl trimethylammonium bromide (CTAAgNP), poly(vinylpyrrolidone) (PVPAgNP), poly-L-lysine (PLLAgNP), and bovine serum albumin (BSAAgNP)) on neural stem cells (NSC). For toxicity evaluations, cell viability and DNA damage in NSC were assessed using CCK-8 and alkaline comet assays, respectively. Significant decrease in cell viability after 24 h exposure for AOTAgNP, CTAAgNPs and PVPAgNPs was observed at 3 mg/L AgNP concentration, while PLLAgNPs and BSAAgNPs induced significant toxicity at concentration of 10 mg/L. Significant DNA damage was observed at 0.1 mg/L of BSAAgNPs, 0.5 mg/L of CTAAgNPs and PLLAgNPs, and 1 mg/L of PVPAgNPs and AOTAgNPs. Our results imply that much lower concentration of AgNPs is able to induce cyto- and genotoxicity in NSC compared to published results on other types of mammalian cells. (Supported by EU FP7 grant GlowBrain: REGPOT-2012-CT2012-316120.)

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Temeljne medicinske znanosti

POVEZANOST RADA