Naslov
Bacterial microflora isolated from gastrointestinal tract of loggerhead sea turtles (Caretta caretta) and their antibiotic resistance

Autori
Zinaić, Elena ; Horvatek Tomić, Danijela ; Gobić, Karin ; Lukač, Maja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of abstract - The 6th International Congress "Veterinary Science and Profession" / Horvatek Tomić, D ; Severin, K ; Slavica, A - Zagreb : Veterinarski fakultet Sveučilišta u Zagrebu, 2015, 64-64

Skup
Šesti međunarodni kongres Veterinarska znanost i struka

Mjesto i datum
Zagreb, Hrvatska, 01.10.2015. - 02.10.2015

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
sea turtles; microflora; gastrointestinal tract

Sažetak
The information on normal gastrointestinal bacterial flora and antibiotic resistance in loggerhead sea turtles (Caretta caretta) is very scarce. The aim of this study was to get the better insight of its composition. For that purpose, oral cavity and cloacal swabs were taken from 17 animals situated at Marine rescue centre Pula. All the samples were plated on nutrient and brilliant green agars and incubated 24 hours at room temperature. After the incubation, all grown bacteria were further identified according to their morphology, Gram staining, catalase and oxidase tests and also by API commercial strips. Antimicrobial resistance was determined by disk diffusion method. From oral cavity, 13 different bacterial species were isolated, 9 gram negative and 4 gram positive. Most common bacteria from oral cavity were Schewanella putrefaciens, followed by Escherichia coli and Micrococcus luteus. From cloaca 15 different bacteria were isolated, 12 gram negative and 3 gram positive species. Most common bacteria isolated from cloaca were Escherichia coli, Proteus vulgaris and Schewanella putrefaciens, followed by Aeromonas salmonicida and Micrococcus sp.. All the other isolated bacteria such as Citrobacter braaki, Citrobacter freundi and, Pseudomonas fluorescens were only randomly isolated from cloaca. All identified bacteria were resistant to amoxicilin, amoxicilin clavulanate and lincomicin. Seven other tested antibiotics have shown a wide range of sensitivity. Most of the isolated bacteria belongs to ubiquitous microorganisms specific for marine environment. However, some of these bacteria were also described as pathogenic for immunocompromised animals and humans. This results can be of interest in the treatment of injured and stressed animals in rescue centers and for the people in closer contact with those animals during their rehabilitation. Most of isolated gram negative bacteria are considered to be opportunistic pathogens as they can cause clinical diseases in immunocompromised animals, but also in humans.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA