Naslov
Aromatic N‐substituted 2‐hydroxyiminoacetamide oximes: preparation, enantioseparation, and interactions with cholinesterases

Autori
Šinko, Goran ; Maraković, Nikola ; Knežević, Anamarija ; Vinković, Vladimir ; Kovarik, Zrinka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
12th International Meeting on Cholinesterases and 6th Paraoxonase Conference, Elche, Španjolska, Program / - , 2015, 99-100

Skup
12th International Meeting on Cholinesterases and 6th Paraoxonase Conference

Mjesto i datum
Elche, Španjolska, 27.09.2015. - 02.10.2015

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
reactivation; cycloadition

Sažetak
We designed and prepared four novel oxime compounds with an N‐substituted 2‐hydroxyiminoacetamide scaffold in order to test their interaction with acetylcholinesterase (AChE) or butyrylcholinesterase (BChE) and their efficacy in reactivation of phosphylated ChEs. 1‐phenylallylamine was prepared from cinnamyl alcohol in an Overman reaction and an azide group was introduced via a three‐step process. The azide group enabled us to prepare more elaborate structures by the copper‐catalysed azide‐alkyne cycloaddition. N‐substituted 2‐ hydroxyiminoacetamides (1 – 4) differ in their peripheral site binding moiety, which ranges from an azide group to functionalized heterocycles. We separated the enantiomers of N‐substituted 2‐hydroxyiminoacetamides using chiral HPLC with high enantiomeric excess (88 % to 99 %). The absolute configuration of the enantiomers was determined by comparing their retention times with N‐substituted 2‐hydroxyiminoacetamides prepared from (S)‐1‐phenyl‐allylamine. All four racemic compounds reversibly inhibited BChE and AChE with inhibition constants ranging from 10 μmol/L to 160 μmol/L and from 30 μmol/L to 900 μmol/L, respectively. All of the compounds displayed a higher preference for binding to BChE, which indicates them as possible candidates for continuous scavenging of organophosphates as BChEoxime pairs. Our preliminary reactivation study on paraoxon‐inhibited BChE shows a reactivation potential for these compounds. We expect that an asymmetrical centre within the molecule will have a beneficial role in the reactivation of cholinesterases inhibited by OP compounds. This work was supported in part by the Croatian Science Foundation (project 4307).

Izvorni jezik
Engleski

Znanstvena područja
Kemija

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