Pregled bibliografske jedinice broj: 779849
Cryopreservation and cryotherapy of grapevine (Vitis vinifera L.)
Cryopreservation and cryotherapy of grapevine (Vitis vinifera L.) // Vitis, 54 (2015), S.I.; 247-251 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 779849 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Cryopreservation and cryotherapy of grapevine (Vitis vinifera L.)
Autori
Marković, Zvjezdana ; Preiner, Darko ; Stupić, Domagoj ; Andabaka, Željko ; Šimon, Silvio ; Vončina, Darko ; Maletić, Edi ; Karoglan Kontić, Jasminka ; Chatelet, Philippe ; Engelmann, Florent
Izvornik
Vitis (0042-7500) 54
(2015), S.I.;
247-251
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
grapevine ; cryopreservation ; cryotherapy ; genetic stability
Sažetak
This study aimed at testing the efficiency of a droplet-vitrification cryopreservation protocol in eliminating selected grapevine viruses. The cryopreservation protocol led to approximately 50 % recovery with cultivar 'Portan' and five international cultivars tested, but very low recovery was noted with Croatian cultivars. GFLV and GLRaV-3, two (economically important grapevine) viruses were eliminated in a high percentage (up to 100 %) of plants regenerated from cryopreserved shoot tips. Virus sanitation was observed as well in samples before liquid nitrogen exposure. Genetic stability of plants regenerated after cryopreservation was studied using AFLP markers. Polymorphic fragments were observed in non-cryopreserved and cryopreserved samples treated with PVS2 solution, the number of which increased with increasing durations of exposure to PVS2 solution.
Izvorni jezik
Engleski
POVEZANOST RADA
Ustanove:
Institut za jadranske kulture i melioraciju krša, Split,
Institut za poljoprivredu i turizam, Poreč,
Agronomski fakultet, Zagreb
Profili:
Darko Vončina
(autor)
Zvjezdana Marković
(autor)
Jasminka Karoglan-Kontić
(autor)
Edi Maletić
(autor)
Silvio Šimon
(autor)
Željko Andabaka
(autor)
Domagoj Stupić
(autor)
Darko Preiner
(autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus