Naslov
Antioxidative properties and GC-MS analyses of Croatian native propolis for implementation in veterinary medicine

Autori
Starčević, Kristina ; Hranjec, Marijana ; Zorić, Ivana ; Brozić, Diana ; Mašek, Tomislav ; Radin, Lada ; Radić, Božo ; Šuran, Jelena

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Days of Veterinary Medicine 2015 / Pendovski, Lazo - Skopje, 2015, 46-46

Skup
Days of Veterinary Medicine 2015

Mjesto i datum
Struga, Sjeverna Makedonija, 24.09.2015. - 26.09.2015

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
bees; propolis; antioxidative properties; veterinary medicine; gas chromatography

Sažetak
Introduction: The aim of this study was to evaluate antioxidative properties and chemical composition of Croatian native propolis (CNP) as a part of the project: „Intramammary propolis formulation for prevention and treatment of mastitis in dairy ruminants”. Propolis is collected by honey bees from various plant sources and its composition and properties depends on geographic region and extraction procedure. This extraction process should remove the inert material and preserve the polyphenolic (flavonoid and other phenolic compounds) fraction, which is considered to contribute more to the observed healing effects than the other propolis constituents. The influence of different extraction methods and solvents on total phenolic compounds, antioxidative capacity and chemical composition of CNP extracts were in focus of this study. Material and methods: Three different methods of extraction were used: maceration, reflux, and microwave-assisted extraction (MAE), as well as three different aqueous solutions of ethanol. Total polyphenol content and antioxidant activities were evaluated for all CNP extracts using respectively Folin-Ciocalteu and 2, 2-diPhenyl-1-PicrylHydrazyl (DPPH). The reduction of this radical by an antioxidant compound results in a decrease in absorbance and is proportional to the number of electrons absorbed, indicating the antiradical capacity of the substances in study. Afterwards, extracts were analysed by GC-MS to quantify chemical compounds responsible for differences in antioxidative capacity. Results: Propolis extracts showed differences in antioxidative capacity determined by DPPH method with the best results for 2 hours, room temperature and ethanol/water ratio 70:30. Extracts also differed in quantity and composition of different compounds responsible for antioxidative properties and biological activity determined by GC-MS. Conclusions: Extraction procedure as well as ratios of solvents has effects on composition of phenolic acids and esters as well as flavonoids and medicinal properties of propolis. All extracts of CNP showed respectable antioxidant activities in comparison to standards (trolox, vitamin C). These data should be considered when using propolis in veterinary medicine.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA