Naslov
Genome stability enzymes are essential for building CRISPR-Cas immunity in bacteria

Autori
Ivančić-Baće, Ivana ; Cass, Simon ; Stephen, Wearne ; Bolt, Edward L.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
Molecules of Life / Kos, Janko ; Poklar Ulrih, Nataša - Ljubljana : Slovenian Biochemical Society, 2015, 197-197

ISBN
978-961-93879-0-0

Skup
FEBS3+ Meeting and 11th Meeting of the Slovenian Biochemical Society

Mjesto i datum
Portorož, Slovenija, 16.09.2015. - 19.09.2015

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
CRISPR-Cas; RecG; PriA; PolA; E. coli

Sažetak
CRISPR-Cas is a prokaryotic immune system built from capture and integration of invader DNA into CRISPR loci by Cas1 and Cas2 proteins, termed "Adaptation". In E. coli, Cascade-Cas3 degrades invader DNA to enact immunity, termed "Interference". Adaptation can be stimulated by interference (primed), or can be independent of interference (naive). We identified that host genome stability enzymes are required for adaptation ; primed adaptation requires RecG and PriA, naive adaptation requires RecB and both types require DNA polymerase I (PolA). Analysis of recG and priA phenotypes indicates interplay between primed adaptation , blocked replication fork and R-loop processing. We further show that Cas1 and Cas2 proteins specifically target substrates mimicking blocked forks. A model is proposed for DNA capture enabled by RecG, PriA and Cas3, or by RecB, according to different types of compromised DNA replication. PolA is proposed to synthesize DNA to fill single strand gaps during capture or integration.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA