Naslov
Influence of in situ formed hydoxyapatite content on freeze-gelled chitosan/hydroxyapatite scaffolds

Autori
Gallego Ferrer, Gloria ; Rogina, Anamarija ; Rico, Patricia ; Ivanković, Marica ; Ivanković, Hrvoje

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
XLII Congress of the European Society for Artificial Organs: The International Journal of Artificial Organs / Remuzzi, Andrea ; Schima, Heinrich - , 2015, 409-409

Skup
XLII Congress of the European Society for Artificial Organs

Mjesto i datum
Leuven, Belgija, 02.09.2015. - 05.09.2015

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Chitosan; Hydroxyapatite; Scaffold; Compression test; MC3T3-E1 differentiation; Osteogenic markers
(chitosan; hydroxyapatite; scaffold; compression test; MC3T3-E1 differentiation; osteogenic markers)

Sažetak
Tissue engineering requires suitable biocompatible scaffolds that can mimic natural bone tissue for cell seeding and neotissue growth. The aim of this research is to synthesize nano-structured composite biomaterials with highly porous structure on the basis of biodegradable polymer chitosan (CS) and in situ formed hydroxyapatite (HA) as a bioactive ceramic. Wet precipitation method has been used for in situ HA formation within chitosan solution, while highly porous structure has been obtained by freeze-gelation technique. The influence of in situ HA weight ratio on scaffold’s composition and microstructure was investigated by FTIR and XRD identification and SEM imaging. Mechanical properties of different CS/HA scaffolds were determined in physiological conditions. Cytotoxicity test of composite scaffolds was carried out by MTS assay, while osteogenic property was evaluated by in vitro culture of MC3T3-E1 preosteoblasts. FTIR and XRD identification confirmed in situ formation of HA as well as the influence of HA precursor’s fraction on mineralogical composition of scaffolds. Freeze-gelation has shown to be optimal technique for producing highly porous structure with good pore interconnectivity. Negative cytotoxicity assay indicated no harmful effect and good biocompatibility. Mechanical testing shows higher Young’s modulus with lower in situ HA weight ratio. Cell culture confirmed cell viability during 14 days and indicates MC3T3-E1 cells differentiation on different CS/HA scaffolds. In situ precipitation has shown to be favourable method for nano-structured chitosan/HA biomaterial formation. High porosity and good pore interconnectivity of prepared scaffolds are important factors for cell and nutrient transport and new tissue formation. Positive osteogenic signal of composite scaffolds indicates a potential application in bone tissue repair.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Kemijsko inženjerstvo, Temeljne tehničke znanosti

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