Naslov
Long Read RNA-seq Mapper

Autori
Marić, Josip

Vrsta, podvrsta i kategorija rada
Ocjenski radovi, diplomski rad, diplomski

Fakultet
Fakultet elektrotehnike i računarstva

Mjesto
Zagreb

Datum
03.06

Godina
2015

Stranica
62

Mentor
Šikić, Mile

Ključne riječi
RNA sequencing; BBMap; precise alignment

Sažetak
RNA sequencing is a technology that uses next generation sequencing tools for transriptome analysis. Bioinformatics tools used are used to analyze RNA sequences to reveal a snapshot of RNA presence and quantity from a genome at a given time which is important for interpreting the functional elements of the genome and understanding development of diseases. This thesis presents a tool built from the BBMap aligning method that is used in mapping long reads with high error rate to the reference genome. Improvements of the developed method are also presented and evaluated. Results are analyzes and compared to the BBMap tool. This paper explains the aligning method used by BBMap and shows that it can be recreated in such manner that it produces highly precise alignment results.

Izvorni jezik
Engleski

Znanstvena područja
Računarstvo

POVEZANOST RADA