Pregled bibliografske jedinice broj: 77301
Macromolecular Architecture in Eukaryotic Cells Visualized by Cryoelectron Microscopy
Macromolecular Architecture in Eukaryotic Cells Visualized by Cryoelectron Microscopy // Science, 298 (2002), 5596; 1209-1213 doi:10.1126/science.1076184 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 77301 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Macromolecular Architecture in Eukaryotic Cells Visualized by Cryoelectron Microscopy
Autori
Medalia, Ohad ; Weber, Igor ; Frangakis, Achilleas S. ; Nicastro, Daniela ; Gerisch, nther ; Baumeister, Wolfgang
Izvornik
Science (0036-8075) 298
(2002), 5596;
1209-1213
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
electron tomography ; Dictyostelium cells ; actin cytoskeleton ; eukaryotic cells
Sažetak
Electron tomography of vitrified cells is a noninvasive three-dimensional imaging technique that opens up new vistas for exploring the supramolecular organization of the cytoplasm. We applied this technique to Dictyostelium cells, focusing on the actin cytoskeleton. In actin networks reconstructed without prior removal of membranes or extraction of soluble proteins, the cross-linking of individual microfilaments, their branching angles, and membrane attachment sites can be analyzed. At a resolution of 5 to 6 nanometers, single macromolecules with distinct shapes, such as the 26S proteasome, can be identified in an unperturbed cellular environment.
Izvorni jezik
Engleski
Znanstvena područja
Biologija
POVEZANOST RADA
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE
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