Naslov
VALIDATION OF TWO PRESUMPTIVE TESTS FOR BLOOD

Autori
Makar, Adela ; Merkaš, Siniša ; Uvodić, Petra ; Kozar-Filić, Jelena ; Ortynski, Nataša ; Crnjac, Josip ; Papić, Sunčica ; Popović, Maja ; Boričević, Ivana ; Abramović, Marko Krešimir ; Mršić, Gordan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
9th ISABS, Book of abstracts / - , 2015, 115-115

Skup
9th ISABS Conference on Forensic, Anthropologic Genetics and Mayo Clinic Lectures in Individualized Medicine

Mjesto i datum
Bol, Hrvatska, 22.06.2015. - 26.06.2015

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Forensics ; Genetics ; Validation ; Combur ; Hemastix

Sažetak
Combur3 Test® E (Roche Diagnostics GmbH, Germany) and Hemastix® (Siemens Healthcare Diagnostics Inc, USA) are used as presumptive tests for blood. In this study, sensitivity and specificity of two tests was examined. Dilutions of blood (from 1:2 to 1:1 000 000) was tested with both tests. Blood on six different substrates was tested directly and after exposure to environemental conditions for 7 days. Additionally, the effect of detergent, bleach and stain removal on white cotton textile with blood was tested. Many common household and chemical substances, as well as substrates that are frequently seen in routine forensic casework, were tested for false positive reaction. Blood samples were tested for DNA to determine whether the presumptive tests, environemental conditions and chemicals damaged or destroyed DNA. Short tandem repeats (STRs) analysis was performed using AmpFlSTR NGM kit (Applied Biosystems, USA). Both tests had a sensitivity of 1:25 000. No effect of different substrates and chemicals on testing results was detected. DNA was recovered and amplified from blood diluted 1:10 000 and from all other blood samples that were tested. Many common household and natural substances, chemical substances and substances which can be mistaken for blood that were tested showed false positive results with both tests.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

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