Naslov
Animal hair as a source of human DNA

Autori
Špoljarić, Daniel ; Ratko, Martina ; Lozančić, Mateja ; Rožić, Sara ; Sukser, Viktorija ; Crnjac, Josip ; Merkaš, Siniša ; Papić, Sunčica ; Jurak, Matea ; Mršić, Gordan ; Popović, Maja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
9th ISABS, Book of abstracts / - , 2015, 114-114

Skup
9th ISABS Conference on Forensic, Anthropologic Genetics and Mayo Clinic Lectures in Individualized Medicine

Mjesto i datum
Bol, Hrvatska, 22.06.2015. - 26.06.2015

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Forensics ; Genetics ; Touch DNA ; Chelex ; EZ1 Investigator

Sažetak
Animal traces are becoming more frequent object of investigation in different forensic and legal cases. The most common animals that can be found in these cases are pets or domesticated animals, such as dogs, cats, parrots, sheep’s etc. In this study, hair samples from 4 different breeds of dogs and sheep’s were obtained. Human DNA samples from three different persons were deposited by rubbing the animal hair samples. For isolation of human DNA two different methods were used, Chelex®100 method and EZ1 Investigator Kit. Quantification of DNA was performed using Quantifiler® Human DNA Quantification Kit and Investigator Quantiplex kit, respectively. Amplification was performed using AmpFlSTR®NGMTM PCR Amplification Kit. Only male partial or full DNA profiles were obtained from animal hair samples. Results pointed out that Chelex®100 shouldbe a preferable method for isolation of human DNA when working with these kinds of forensic samples.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

POVEZANOST RADA