Naslov
Analysis of 12 X- chromosome STR LOCI with reduced PCR sample volume

Autori
Crnjac, Josip ; Merkaš, Siniša ; Rožić Sara ; Sukser, Viktorija ; Špoljarić, Daniel ; Popović, Maja ; Anđelinović, Šimun ; Primorac, Dragan ; Mršić, Gordan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
9th ISABS, Book of abstracts / - , 2015, 112-112

Skup
9th ISABS Conference on Forensic, Anthropologic Genetics and Mayo Clinic Lectures in Individualized Medicine

Mjesto i datum
Bol, Hrvatska, 22.06.2015. - 26.06.2015

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Forensics ; DNA analysis ; Investigator Argus X-12 ; X chromosome ; Genetics

Sažetak
STR markers on the X chromosome have emerged as additional tools in forensics, in missing person cases and parent-child relationships. Qiagen's Investigator® Argus X-12 kit for X- chromosome STR's, which analyses 12 loci (DXS10103, DXS8378, DXS7132, DXS10134, DXS10074, DXS10101, DXS10135, DXS7423, DXS10146, DXS10079, HPRTB, DXS10148) and amelogenin is currently being used. Following manufactures analysis protocol, obtained results from female samples were exhibiting spikes and pull- ups in electrophoresis, which were not found in male samples. Since the standard procedure requires samples to be normalized to concentrations of 1ng/µL of DNA before conducting PCR amplification, results from both male and female samples should be coherent. Due to observed spikes and pull-ups in female samples we diluted female and male normalized samples to 0.5ng/µL of DNA before setting up PCR reaction. Obtained results with diluted samples showed no spikes and pull-ups in female DNA profiles, while allele drop- out was observed in male DNA profiles. Thus we recommend normalization of female samples s to 0.5ng/µL of DNA rather than 1ng/µL of DNA asproposed by manufacturer.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

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