Naslov
Antibody-based donor-acceptor spatial reconfiguration in decorated lanthanide-doped nanoparticle colloids for the quantification of okadaic acid biotoxin

Autori
Stipić, Filip ; Burić, Petra ; Jakšić, Željko ; Pletikapić, Galja ; Dutour Sikirić, Maja ; Zgrablić, Goran ; Frkanec, Leo ; Lyons, Daniel Mark

Izvornik
Colloids and surfaces. B, Biointerfaces (0927-7765) 135 (2015); 481-489

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Biosensor; diagnostic; nanoparticle; lanthanide; immunoassay; point-of-care

Sažetak
With the increasing movement away from the mouse bioassay for the detection of toxins in commercially harvested shellfish, there is a growing demand for the development of new and potentially field-deployable tests in its place. In this direction we report the development of a simple and sensitive nanoparticle-based luminescence technique for the detection of the marine biotoxin okadaic acid. Photoluminescent lanthanide nanoparticles were conjugated with fluorophore-labelled anti-okadaic acid antibodies which, upon binding to okadaic acid, gave rise to luminescence resonance energy transfer from the nanoparticle to the organic fluorophore dye deriving from a reduction in distance between the two. The intensity ratio of the fluorophore : nanoparticle emission peaks was found to correlate with okadaic acid concentration, and the sensor showed a linear response in the 0.37–3.97 μM okadaic acid range with a limit of detection of 0.25 μM. This work may have important implications for the development of new, cheap and versatile biosensors for a range of biomolecules and that are sufficiently simple to be applied in the field or at point-of-care.

Izvorni jezik
Engleski

Znanstvena područja
Fizika, Kemija, Biologija

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