Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 770220

Modulation of the cell wall of yeast Candida albicans with berberine


Bobnjarić, Ivan; Vlainić, Josipa; Zorić, Nataša; Kosalec, Ivan
Modulation of the cell wall of yeast Candida albicans with berberine // Knjiga sažetaka 5. hrvatskog kongresa farmacije / Zorc, Branka (ur.).
Rovinj, 2015. str. 163-163 (poster, domaća recenzija, sažetak, ostalo)


CROSBI ID: 770220 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Modulation of the cell wall of yeast Candida albicans with berberine

Autori
Bobnjarić, Ivan ; Vlainić, Josipa ; Zorić, Nataša ; Kosalec, Ivan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
Knjiga sažetaka 5. hrvatskog kongresa farmacije / Zorc, Branka - Rovinj, 2015, 163-163

ISBN
978-953-7897-05-5

Skup
5. hrvatski kongres farmacije s međunarodnim sudjelovanje

Mjesto i datum
Rovinj, Hrvatska, 21.05.2015. - 24.05.2015

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
berberine; Candida albicans; ergosterol; cell wall

Sažetak
Candida albicans is endogenous opportunistic yeast that does not cause infections in healthy individuals but rather cause superficial to systemic infections in immuno-compromised individuals. The use of fungistatic drugs and the lack of -cidal drugs often results in strains that are not susceptible to commonly used antifungals, and/or display multidrug resistance (MDR). Therefore, there is a need to find new antifungals with novel targets. The aim of the present study was to determine the antifungal effects of the isoquinoline alkaloid berberine and to elucidate the mechanism of this effect in C. albicans. Using serial dilution assays we determined the minimal inhibitory concentration (MIC) of berberine against C. albicans. The ergosterol levels were assessed measuring the spectrophotometric absorbance profile between 240 and 300 nm (sterol quantitation method, SQM). To analyse the possible effects of berberine on the cell wall we tested the releasing of the crucial cell content using spectrophotometric measurement at 260/280 nm and 750 nm (corresponding to nucleic acids and proteins). Since secretion of aspartic proteinases is one of the important determinants associated with the pathogenicity of Candida species, we deterimened the effect of berberine on SAP2 activity using secreted aspartyl proteinase (SAP) test. In the present study, we observed that berberine affected cell growth of tested Candida albicans strain at the 17.75 μg/ml indicating its good antifungal properties. In our study, the significant differences in mean ergosterol content were observed in berberine treated and non-treated group, which were dose and time dependent. Moreover, the results indicate that berberine and positive control (voriconazol) produce the same levels of ergosterol indicating their entanglement in the ergosterol biosynthesis. Moreover, berberine treatment caused the elevated levels of proteins and nucleic acids indicating the releasing of the cell content in the extracellular matrix. Namely, on the basis of these results we assumed that berberine treatment affects the cell membrane permeability and or integrity. In addition, as determined it is indicative that berberine does not inhibit proteinase activity (e.g. SAP2). In summary, the data obtained indicate that berberine inhibit ergosterol biosynthesis. Moreover, according to our results one might assume that berberine binds to the membrane sterols consequently causing aqueous pores formation and the leakage of cellular components what can lead to cell death. Thus, further investigation on the mechanisms of actions on the cell wall is required.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti, Farmacija



POVEZANOST RADA


Ustanove:
Farmaceutsko-biokemijski fakultet, Zagreb,
Institut "Ruđer Bošković", Zagreb

Profili:

Avatar Url Josipa Vlainić (autor)

Avatar Url Ivan Kosalec (autor)

Poveznice na cjeloviti tekst rada:

Pristup cjelovitom tekstu rada

Citiraj ovu publikaciju:

Bobnjarić, Ivan; Vlainić, Josipa; Zorić, Nataša; Kosalec, Ivan
Modulation of the cell wall of yeast Candida albicans with berberine // Knjiga sažetaka 5. hrvatskog kongresa farmacije / Zorc, Branka (ur.).
Rovinj, 2015. str. 163-163 (poster, domaća recenzija, sažetak, ostalo)
Bobnjarić, I., Vlainić, J., Zorić, N. & Kosalec, I. (2015) Modulation of the cell wall of yeast Candida albicans with berberine. U: Zorc, B. (ur.)Knjiga sažetaka 5. hrvatskog kongresa farmacije.
@article{article, author = {Bobnjari\'{c}, Ivan and Vlaini\'{c}, Josipa and Zori\'{c}, Nata\v{s}a and Kosalec, Ivan}, editor = {Zorc, B.}, year = {2015}, pages = {163-163}, keywords = {berberine, Candida albicans, ergosterol, cell wall}, isbn = {978-953-7897-05-5}, title = {Modulation of the cell wall of yeast Candida albicans with berberine}, keyword = {berberine, Candida albicans, ergosterol, cell wall}, publisherplace = {Rovinj, Hrvatska} }
@article{article, author = {Bobnjari\'{c}, Ivan and Vlaini\'{c}, Josipa and Zori\'{c}, Nata\v{s}a and Kosalec, Ivan}, editor = {Zorc, B.}, year = {2015}, pages = {163-163}, keywords = {berberine, Candida albicans, ergosterol, cell wall}, isbn = {978-953-7897-05-5}, title = {Modulation of the cell wall of yeast Candida albicans with berberine}, keyword = {berberine, Candida albicans, ergosterol, cell wall}, publisherplace = {Rovinj, Hrvatska} }




Contrast
Increase Font
Decrease Font
Dyslexic Font