Naslov
Cytokinesis mediated through the recruitment of cortexillins into the cleavage furrow

Autori
Weber, Igor ; Gerisch, G. ; Heizer, C. ; Murphy, J. ; Badelt, K. ; Stock, A. ; Schwartz, J.-M. ; Faix, J.

Izvornik
EMBO Journal (0261-4189) 18 (1999); 586-594

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Sažetak
The fact that substrate-anchored Dictyostelium cells undergo cytokinesis in the absence of myosin II underscores the importance of other proteins in enabling the cleavage furrow to constrict. Cortexillins, a pair of actin-bundling proteins, are required for normal cleavage. They are targeted to the incipient furrow in wild-type and, more prominently, in myosin II-null cells. No other F-actin bundling or cross-linking protein tested is co-localized, Green fluorescent protein fusions show that the N-terminal actin-binding domain of cortexillin I is dispensable and the C-terminal region is sufficient for translocation to the furrow and the rescue of cytokinesis. Cortexillins are suggested to have a targeting signal for coupling to a myosin II-independent system that directs transport of membrane proteins to the cleavage furrow.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA