Naslov
ANTIOXIDANT PROPERTIES OF LOTUS CORNICULATUS L . EXTRACTS

Autori
Fumić, Barbara ; Zovko Končić, Marijana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts MESMAP 2015 / Nazim, Sekeroglu ; Hakan, Cetinkaya - Ankara : Republic of Turkey Ministry of Forestry and Water Affairs General Directorate Of Forestry, Ankara, Turkey, 2015, 463-463

Skup
2nd Mediterranean Symposium on Medicinal and Aromatic Plants

Mjesto i datum
Antalya, Turska, 22.04.2015. - 25.04.2015

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Lotus Corniculatus L.; experimental design; antioxidant activity

Sažetak
In this study, the effect of extraction conditions on antiradical and chelating activity of Lotus corniculatus L. extracts was investigated. The optimum conditions for the extraction were determined using response surface methodology (RSM). Box Behnken design was used to investigate the effects of three variables: ethanol concentration (%), temperature (°C) and pH. Selected variables were coded at three levels and their actual values selected on the basis of preliminary experimental results. Spectrophotometric methods were used for determination of antiradical and chelating activity in extracts. Data was analysed using Design Expert software. The optimal conditions for extraction of compounds with high antioxidant activity were 62% ethanol, 60°C and pH value of 7.3. Extraction was repeated under optimum conditions to check the validity of the model. The experimental EC50 values for antiradical and chelating activity were 87.41 and 109.12 µg /ml while predicted values were 85.1 and 102.6 µg /ml respectively. Experimental values agreed with those predicted within 2, 7% and 6, 4%, thus indicating suitability of the model employed and the success of RSM in optimizing the extraction conditions. Data from the presented results revealed that Lotus corniculatus L. act as an antioxidant agent due to its free radical scavenging and chelating activity.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

POVEZANOST RADA