Naslov
ISOFLAVONE COMPOSITION OF LOTUS CORNICULATUS L. EXTRACTS

Autori
Fumić, Barbara ; Zovko Končić, Marijana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Knjiga sažetaka- 5. Hrvatski kongres farmacije s međunarodnim sudjelovanjem / Zorc, Branka - Zagreb : Hrvatsko farmaceutsko društvo, 2015, 203-203

ISBN
978-953-7897-05-5

Skup
5. Hrvatski kongres farmacije s međunarodnim sudjelovanjem

Mjesto i datum
Rovinj, Hrvatska, 21.05.2015. - 24.05.2015

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Lotus Corniculatus L.; HPLC; isoflavones

Sažetak
Isoflavones are a class of phytoestrogens, plant-derived compounds with estrogenic activity. Numerous clinical studies claim benefits of isoflavones in chemoprevention of breast and prostate cancer, cardiovascular disease and osteoporosis, as well as in relieving postmenopausal symptoms. In this study, composition of isoflavones in different Lotus corniculatus L. extracts was investigated. Upon hydrolysis using hydrochloric acid total content of isoflavone aglycones was determined by high performance liquid chromatography (HPLC). Analysis was run on Agilent 1220 HPLC equipped with variable wavelength detector. Separation was performed on a Agilent Zorbax SB-C18 column, using mobile phases: 0.1 % formic acid in methanol (A) and 0.1% formic acid (B). Elution was performed at a flow rate of 1 ml/min at the temperature of 40 C using gradient method. Isoflavones were identified by the comparison of their retention times with standards acquired commercially. Content of isoflavone aglycones was determined using calibration curves of standards (r2  0, 999). The extractability of compounds significantly increased from aqueous to hydroalcoholic extracts, where genistein was the most abundant isoflavone compound (3.2 g/ ml). Daidzein and glycitein were detected in concentrations of 1.32 and 2.66 g/ ml respectively. The study demonstrates that L. corniculatus L. areal parts possess a promissing potential as a source of these health promoting isoflavone compounds.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

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