Naslov
INFLUENCE OF EXTRACTION TECHNIQUES ON GC-MS SCREENING RESULTS

Autori
Mandić, Dario ; Mandić, Sanja ; Horvat, Vesna ; Majetić-Cetina, Neda

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Toxicology Letters 2008 ; 180, Supplement, Abstracts of the 45th Congress of the European Societies of Toxicology (EUROTOX), Rhodes, Greece, 5–8 October 2008 / - Amsterdam : Elsevier, 2008, S136-S136

Skup
45th Congress of the European Societies of Toxicology (EUROTOX), Rhodes, Greece, 5–8 October 2008

Mjesto i datum
Rodos, Grčka, 05.10.2008. - 08.10.2008

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
extraction ; gc-ms ; screening ; toxicology

Sažetak
Gas chromatography coupled with mass spectrometry (GC-MS) is often used as a confirmatory technique for positive results obtained with immunoassay screening tests, but it can also be used as a screening method in clinical and forensic toxicology. Because of different reasons (amount of sample available, urgency, cost of analysis, etc.), laboratory is often in position to perform just one extraction and screening per sample. Influence of different extraction procedures on results is usually overlooked, but it can have great impact on obtained results, both quantitatively and qualitatively. We prepared four different levels of commercial urine toxicology control samples Liquichek (BIO-RAD), containing 12 drugs-ofabuse using three different extraction procedures: liquid– liquid extraction system TOXI-TUBES A (VARIAN), liquid–liquid extraction system Chem Elut (VARIAN) and solid phase extraction system based on Amberlite XAD2 polymeric adsorbent columns (Sigma–Aldrich). All extracts were analyzed with GC-MS (SHIMADZU GCMS-QP5050A). Obtained results are presented and influence of extraction procedure on GC-MS screening results is evaluated for every extraction procedure.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

POVEZANOST RADA