Naslov
Mechanisms of vascular responses to changes in flow in cerebral resistance arteries of healthy Sprague-Dawley rats

Autori
Ćosić, Anita ; Jukić, Ivana ; Čavka, Ana ; Novak, Sanja ; Mihalj, Martina ; Drenjančević, Ines

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Journal of Vascular Research 52(suppl 1):1-88 / Rossi, Marco ; Koller, Akos ; Dulak, Jozef - : Karger Publishers, 2015, 72-73

Skup
Joint Meeting of the European Society of Microcirculation (ESM) and European Vascular Biology Organisation (EVBO) 2015

Mjesto i datum
Pisa, Italija, 03.06.2015. - 06.06.2015

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
flow induced dilation; cerebral resistance arteries; rats

Sažetak
Aim: We previously observed that flow induced dilatation (FID) in middle cerebral arteries (MCA) of healthy rats is mediated mainly by NO. However, the metabolites of COX1, 2 and EETs could not be excluded in FID. The aim of this study was to assess potential contribution of other vasodilators in FID. Methods: 12-weeks old healthy male Sprague-Dawley rats were anesthetized with ketamin-chloride (75 mg/kg) and midazolam (2.5 mg/kg) prior to decapitation.MCA were isolated and cannulated for vascular reactivity measurements in response to stepwise increase in pressure (Δ10- Δ100) in the presence of different combination of inhibitors INDO, L-NAME and MS-PPOH (N=4-6 per combination).Two-way ANOVA was used for data analysis ; p<0.05 considered significant. Results: FID was reduced at each pressure gradient in the presence of all 3 inhibitors together compared to baseline. Combination of any 2 inhibitors significantly reduced FID compared to basal values but not compared to any individual inhibitor. All 3 inhibitors reduced FID compared to any individual inhibitor but at different pressure gradient(INDO vs 3 nhibitors Δ40 and Δ100 ; INDO vs 3 inhibitors at Δ20, Δ40 and Δ60 ; MS- PPOH vs 3 inhihitors at Δ20 and Δ40). Conclusions: The results confirmed redundancy of mechanisms mediating FID. Beside NO, metabolites of COX1, 2 and EETs also contribute to FID in MCA of healthy rats.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA