Naslov
Antibacterial activity of Pinus pinaster bark extract and its components against multidrug-resistant clinical isolates of Acinetobacter baumannii

Autori
Ćurković-Perica, Mirna ; Hrenović, Jasna ; Kugler, Nuša ; Goić-Barišić, Ivana ; Tkalec, Mirta

Izvornik
Croatica chemica acta (0011-1643) 88 (2015), 2; 133-137

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
antimicrobials; aqueous extract; bacteria; multidrug-resistant pathogens; natural compounds

Sažetak
The aim of this research was to test the antibacterial activity of Pinus pinaster aqueous bark extract (PABE) and its basic components against multidrug-resistant isolates of Acinetobacter baumannii belonging to European clone I and II, isolated previously form the hospital environment. The minimum bactericidal concentration of PABE against both clones of A. baumannii was 200 mg ml-1, while lower concentrations showed high antibacterial activity. After 24 h of treatment with 100, 50 or 10 mg ml-1 of extract, the reduction in the number of A. baumannii isolates belonging to European clone I and II was 85.8±2.5%, 78.5±1.1%, 66.3±2.5% and 90.2±1.7%, 78.6±1.2%, 69.8±0.7%, respectively. Several basic components: caffeic acid, catechin, epicatechin, gallic acid and vanillin, detected in the extract by high performance liquid chromatography, contributed to the antibacterial activity of the extract against both clones of A. baumannii. However, the antibacterial activity of extract was higher than that of each tested basic component suggesting that proanthocyanidins, which were present in quite a large amount in the extract, might have also contributed to the activity of the extract. Antibacterial activity of PABE against A. baumannii reveals that complex and inexpensive natural product might be useful in combat against naturally competent bacteria that easily acquire resistance against antibiotics.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

POVEZANOST RADA