Naslov
EXPRESSION OF WATER CHANNEL AQP1 IN PIG KIDNEYS IS GENDER-DEPENDENT

Autori
Brzica, Hrvoje ; Žura Žaja, Ivona ; Ljubojević, Marija ; Herak- Kramberger, Carol M. ; Del Vechio, Igor ; Milinković-Tur, Suzana ; Ćurković, Snježana ; Vuković, Snježana ; Špiranec, Katarina ; Bačić, Goran ; Maćešić, Nino ; Budinšćak, Zvonimir ; Sabolić, Ivan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of abstracts of the 2nd International Scientific Meeting of Anatomy and Physilogy Fundementals of medicine / Vilić, Marinko ; Lucić, Hrvoje - Zagreb : Veteriranrski fakultet Sveučilišta u Zagrebu, 2014, 31-31

ISBN
987-953-6062-98-0

Skup
2nd International Scientific Meeting of Anatomy and Physilogy Fundementals of medicine

Mjesto i datum
Zagreb, Hrvatska, 16.06.2014. - 17.06.2014

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Nije recenziran

Ključne riječi
aquaporin 1; pig kidneys; gender dependent

Sažetak
Water channel 1 (AQP1), a small integral membrane protein expressed in the basolateral (BLM) and apical (AM) cell membranes of proximal tubule and thin descending limb of Henle, mediates ≥ 65% of water reabsorption in the mammalian kidneys. The expression of AQP1 has been extensively studied in various rat and human organs, while the corresponding data in pig are mostly restricted to the reproductive tract. The aim of this work was to determine whether the expression of AQP1 in pig kidneys is influenced by sex hormones. Swedish landrace pigs (10 of each sex) were either sham operated (5 males (M) and 5 female (F)) or gonadectomized (5 M and 5 F) at the age of 4 weeks and sacrificed at the age of 10 months. Kidney tissue samples were collected and tested for AQP1 protein expression by Western blotting of isolated membranes and by immunostaining of tissue cryosections using specific polyclonal antibodies. With both methods, the expression of AQP1 protein along the nephron was strongly gender-depend, being in sham operated F˃M. Castration had no effect, while ovariectomy downregulated the expression approximately to the levels in sham operated M. These data indicate that the expression of AQP1 in pig kidneys is stimulated by estrogens. This is in contrast to the situation in rats, where the renal AQP expression is male dominant due to androgen stimulation. Opposite gender- and sex hormone-driven AQP1 protein expression in rat and pig kidneys proves the existence of species differences regarding this water channel.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina, Filozofija

POVEZANOST RADA