Naslov
Application of alkaline elution, fast micromethod and flow cytometry in detection of marine contamination

Autori
Bihari, Nevenka ; Hamer, Bojan ; Jakšić, Željko ; Fafanđel, Maja ; Mičić, Milena ; Batel, Renato

Izvornik
Cellular and molecular biology (0145-5680) 48 (2002), 4; 373-377

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
DNA damage; environmental contamination; alkaline filter elution; flow cytometry; Fast micromethod

Sažetak
DNA damage is an inescapable aspect of life in the biosphere. The presented investigations were an attempt to examine the response of a DNA damage as a biomarker of environmental quality in the mussels mytilus galloprovincialis sampled at differently contaminated areas of Istrian coast, Northern Adiatic. The investigation were performed in order to get information about the genotoxic risk for marine organisms exposed to mixed environmental pollution, as well as the information about the presence of unknown mixture of genotoxic contaminants in the marine environment.Types of DNA damage detected are alkali-labile sites and single-strand breaks measured by Fast micromethod, interstrand cross-links and DNA protein crosslinks by alkaline filter elution and cell cycle disturbation by flow cytometry. The applicability of all three methods for marine quality control is discussed.

Izvorni jezik
Engleski

Znanstvena područja
Geologija

POVEZANOST RADA