Naslov
Characterization and In vitro Biocompatibility Assessment of Newly Developed Supramolecular Gel

Autori
Ferhatović Hamzić, Lejla ; Pospišil, Tihomir ; Frkanec, Leo ; Lovrić, Marija ; Gajović, Srećko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
XXIV. Hrvatski skup kemičara i kemijskih inženjera, Knjiga sažetaka / Š. Ukić ; T. Bolanča - Zagreb : Hrvatsko društvo kemijskih inženjera i tehnologa (HDKI), 2015, 73-73

ISBN
978-953-6894-54-3

Skup
XXIV. Hrvatski skup kemičara i kemijskih inženjera

Mjesto i datum
Zagreb, Hrvatska, 21.04.2015. - 24.04.2015

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
supramolecular gel; characterization; in vitro; biocompatibility

Sažetak
Hydrogel biomaterials, closely mimicking the three-dimensional extracellular matrix, are considered to be an ideal material for cell and tissue scaffolding applications. The dynamic nature of the noncovalent hydrogels fibril network allows the hydrogel material to spontaneously adjust to the surrounding environment and the cells to migrate through the matrix comparing to covalent hydrogels whose pores are chemically constrained and relatively inflexible. A new type of such noncovalent supramolecular gel, the self- assembling peptide (SAP) hydrogel has been designed and synthesized. Spontaneous assembly of low molecular weight tripeptides that form extensive hydrogen bonds and π-π stacking under physiological conditions results the formation of hydrogel fibrous material. Series of experiments have been performed in order to assess in vitro biocompatibility of the novel material. HEK293T cells have been used as a reproducible model cell line and were cultured following established proliferation protocols. Survival and proliferation of HEK293T cells encapsulated within SAP gel have been evaluated by using Live/Dead® Cell Viability assay and PicoGreen DNA quantification assay. Analysis was performed on 0 and 5 days upon encapsulation. Results showed high viability and high proliferation rate of the cells distributed throughout the SAP hydrogel structure. The obtained material was proved to serve as stabile and biocompatible physical support in improving HEK293T cells biological outcome in vitro. Furthermore, these results suggest further development of designed three- dimensional engineered tissue culturing system as well as evaluation of in vitro biocompatibility and bioactivity on the neural stem cells in order to be investigated for brain tissue engineering application. The study was supported by EU FP7 grant GlowBrain (REGPOT–2012–CT2012–316120).

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Temeljne medicinske znanosti

POVEZANOST RADA