Pregled bibliografske jedinice broj: 759232
The mechanism of synergistic effects of arsenic trioxide and rapamycin in acute myeloid leukemia cell lines lacking typical t(15 ; 17) translocation
The mechanism of synergistic effects of arsenic trioxide and rapamycin in acute myeloid leukemia cell lines lacking typical t(15 ; 17) translocation // International journal of hematology, 102 (2015), 1; 12-24 doi:10.1007/s12185-015-1776-2 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 759232 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
The mechanism of synergistic effects of arsenic trioxide and rapamycin in acute myeloid leukemia cell lines lacking typical t(15 ; 17) translocation
Autori
Dembitz, Vilma ; Lalić, Hrvoje ; Ostojić, Alen ; Vrhovac, Radovan ; Banfić, Hrvoje ; Višnjić, Dora
Izvornik
International journal of hematology (0925-5710) 102
(2015), 1;
12-24
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
Arsenic trioxide; Rapamycin; U937; HL-60; CD64
Sažetak
Arsenic trioxide (ATO) has potent clinical activity in the treatment of patients with acute promyelocytic leukemia (APL), but is much less efficacious in acute myeloid leukemia (AML) lacking t(15 ; 17) translocation. Recent studies have indicated that the addition of mammalian target of rapamycin (mTOR) inhibitors may increase the sensitivity of malignant cells to ATO. The aim of the present study was to test for possible synergistic effects of ATO and rapamycin at therapeutically achievable doses in non-APL AML cells. In HL-60 and U937 cell lines, the inhibitory effects of low concentrations of ATO and rapamycin were synergistic and more pronounced in U937 cells. The combination of drugs increased apoptosis in HL-60 cells and increased the percentage of cells in G0/G1 phase in both cell lines. In U937 cells, rapamycin alone increased the activity of mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) and the addition of ATO decreased the level of phosphorylated ERK, Ser473 phosphorylated Akt and anti-apoptotic Mcl-1 protein. Primary AML cells show high sensitivity to growth-inhibitory effects of rapamycin alone or in combination with ATO. The results of the present study reveal the mechanism of the synergistic effects of two drugs at therapeutically achievable doses in non-APL AML cells.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti
POVEZANOST RADA
Projekti:
108-0000000-3455 - Dijagnostika i terapija infekcija kod imunokompromitiranih bolesnika (Vrhovac, Radovan, MZOS ) ( CroRIS)
108-1081347-0173 - Funkcija fosfoinozitol 3-kinaze C2 beta u staničnim jezgrama (Banfić, Hrvoje, MZOS ) ( CroRIS)
108-1081347-1448 - Uloga PLC i Akt u staničnom ciklusu i diferencijaciji leukemija (Višnjić, Dora, MZOS ) ( CroRIS)
Ustanove:
Medicinski fakultet, Zagreb,
Klinički bolnički centar Zagreb
Profili:
Dora Višnjić
(autor)
Radovan Vrhovac
(autor)
Vilma Dembitz
(autor)
Hrvoje Banfić
(autor)
Hrvoje Lalić
(autor)
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE