Naslov
MRNA isolation from archive paraffin-embedded lymphatic tissue specimens for transcription factor expression analysis

Autori
Korbler, Tajana ; Gršković, Marica ; Kušić, Borka ; Dominis, Marija ; Antica, Mariastefania

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Scandinavian Journal of Immunology / Jonsson, R ; Dembic, Z ; Ljunggren, H. G. - Oxford : Wiley-Blackwell, 2002, 534-535

Skup
Scandinavian Society for Immunology 33rd Annual Meeting and Scandinavian Society for Immunology 18th Summer School

Mjesto i datum
Bergen, Norveška, 23.04.2002. - 28.04.2002

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
RNA isolation; paraffin-embedded tissue; transcription factor

Sažetak
Gene activation that underlies lymphoid cell differentiation has been one of the more investigated topics in immunology in the recent years. However, the analysis of these molecular events in lymphoproliferative diseases is often hampered by the lack of fresh material. Most tissues available for routine pathological investigation are formalin-fixed and paraffin-embedded. Gene expression in such specimens could be analysed using reverse transcription of mRNA and polymerase chain reaction (RT-PCR). Therefore we established a method for mRNA isolation from such specimens by modifying the phenol/chloroform extraction method. Given the significance of transcription factors in the human hemopoietic system, we investigated whether mRNA could be successfully isolated from archival tissue, for a comprehensive study on expression of transcription factors in lymphatic tissue

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA