Pregled bibliografske jedinice broj: 758581
The role of sevoflurane and ionising radiation on the level of primary DNA damage measured in blood and different organs of Swiss albino mice by the alkaline comet assay
The role of sevoflurane and ionising radiation on the level of primary DNA damage measured in blood and different organs of Swiss albino mice by the alkaline comet assay // Proceedings of the tenth Symposium of the Croatian Radiation Protection Association / Petrinec, Branko ; Bituh, Tomislav ; Milić, Mirta ; Kopjar, Nevenka (ur.).
Zagreb: Denona, 2015. str. 126-131 (poster, domaća recenzija, cjeloviti rad (in extenso), znanstveni)
CROSBI ID: 758581 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
The role of sevoflurane and ionising radiation on the level of primary DNA damage measured in blood and different organs of Swiss albino mice by the alkaline comet assay
Autori
Benković, Vesna ; Milić, Mirta ; Horvat Knežević, Anica ; Halovanović, Sanja ; Borojević, Nikola ; Oršolić, Nada
Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni
Izvornik
Proceedings of the tenth Symposium of the Croatian Radiation Protection Association
/ Petrinec, Branko ; Bituh, Tomislav ; Milić, Mirta ; Kopjar, Nevenka - Zagreb : Denona, 2015, 126-131
Skup
10th Symposium of the Croatian Radiation Protection Association with international participation
Mjesto i datum
Šibenik, Hrvatska, 15.04.2015. - 17.04.2015
Vrsta sudjelovanja
Poster
Vrsta recenzije
Domaća recenzija
Ključne riječi
sevoflurane ; ionising radiation ; Swiss albino mice ; comet assay
Sažetak
Sevoflurane is general anaesthetic suitable for short surgical procedures due to its quick induction of anaesthesia, maintaining spontaneous breathing frequency and hemodynamic stability of patients. However, it can directly trigger the formation of peroxynitrite, significantly increase intracellular levels of H2O2, peroxide, superoxide anion and nitric oxide in peripheral polymorphonuclear neutrophils 1h after the treatment ; lowering the levels of intracellular glutathione, and increase radiosensitivity of cells also exposed to ionising radiation (IR). We wanted to evaluate the level of sinergistic effect and possible radiosensitivity DNA damage in blood, and different organs of Swiss albino mice after exposure to both sevoflurane (2.4 %, 50:50) and the 1Gy gamma-ray radiation generally used in diagnostic purposes after 0, 2, 6 and 24 hours from the combined treatment with alkaline comet assay. Combined exposure to sevoflurane and IR has demonstrated synergistic effect. Due to metabolising of the sevoflurane, there was different sensitivity between blood, liver, kidney and brain cells.
Izvorni jezik
Engleski
Znanstvena područja
Biologija, Javno zdravstvo i zdravstvena zaštita, Farmacija
POVEZANOST RADA
Projekti:
022-0222148-2137 - Genotoksičnost kemijskih i fizikalnih agensa prirodnog i antropogenog podrijetla (Kašuba, Vilena, MZOS ) ( CroRIS)
119-0000000-1255 - Kemoprevencija rasta tumora polifenolnim sastavnicama (Oršolić, Nada, MZOS ) ( CroRIS)
Ustanove:
Institut za medicinska istraživanja i medicinu rada, Zagreb,
Prirodoslovno-matematički fakultet, Zagreb
Profili:
Nikola Borojević
(autor)
Vesna Benković
(autor)
Mirta Milić
(autor)
Anica Horvat Knežević
(autor)
Nada Oršolić
(autor)