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Pregled bibliografske jedinice broj: 758444

The application of single strand conformation polymorphism (SSCP) analysis in determining Hepatitis E virus intra-host diversity

Černi, Silvija; Prpić, Jelena; Jemeršić, Lorena; Škorić, Dijana
The application of single strand conformation polymorphism (SSCP) analysis in determining Hepatitis E virus intra-host diversity // Journal of virological methods, 221 (2015), 46-50 doi:10.1016/j.jviromet.2015.04.020 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 758444 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
The application of single strand conformation polymorphism (SSCP) analysis in determining Hepatitis E virus intra-host diversity

Autori
Černi, Silvija ; Prpić, Jelena ; Jemeršić, Lorena ; Škorić, Dijana

Izvornik
Journal of virological methods (0166-0934) 221 (2015); 46-50

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
cloning; capsid protein; HEV; quasispecies; SSCP

Sažetak
Genetic heterogeneity of RNA populations influences virus pathogenesis, epidemiology and evolution. Therefore, accurate information regarding virus genetic structure is highly important for both diagnostic and scientific purposes. For Hepatitis E virus (HEV), the causal agent of human hepatitis, the intra-host population structure has been poorly investigated, mainly using the less sensitive RFLP-based approach. The objective of this study was to assess the suitability and the accuracy of single strand conformation polymorphism (SSCP) analysis, a well- established tool in the genetic variation research, for the characterization of HEV quasispecies. The analysis was conducted on 50 clones of five swine isolates and 30 clones of three human HEV isolates. To identify and quantify the sequence variants present in each HEV isolate, 348 bp long fragments of the amplified conserved ORF2 region were separated by cloning. Ten clones per isolate were subjected to SSCP and sequenced in a parallel experiment. The results show a high correlation of SSCP haplotype profiling with the sequencing results, confirming the sensitivity and reliability of this simple, rapid and low cost approach in the characterization of HEV quasispecies.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Veterinarska medicina



POVEZANOST RADA

Projekti:
048-0481186-1183 - Primjena genetičkih analiza u veterinarskoj medicini (Lojkić, Mirko, MZOS ) ( CroRIS)

Ustanove:
Hrvatski veterinarski institut, Zagreb,
Prirodoslovno-matematički fakultet, Zagreb

Profili:

Avatar Url Jelena Prpić (autor)

Avatar Url Silvija Černi (autor)

Avatar Url Lorena Jemeršić (autor)

Avatar Url Dijana Škorić (autor)

Poveznice na cjeloviti tekst rada:

doi www.sciencedirect.com dx.doi.org

Citiraj ovu publikaciju:

Černi, Silvija; Prpić, Jelena; Jemeršić, Lorena; Škorić, Dijana
The application of single strand conformation polymorphism (SSCP) analysis in determining Hepatitis E virus intra-host diversity // Journal of virological methods, 221 (2015), 46-50 doi:10.1016/j.jviromet.2015.04.020 (međunarodna recenzija, članak, znanstveni)
Černi, S., Prpić, J., Jemeršić, L. & Škorić, D. (2015) The application of single strand conformation polymorphism (SSCP) analysis in determining Hepatitis E virus intra-host diversity. Journal of virological methods, 221, 46-50 doi:10.1016/j.jviromet.2015.04.020.
@article{article, author = {\v{C}erni, Silvija and Prpi\'{c}, Jelena and Jemer\v{s}i\'{c}, Lorena and \v{S}kori\'{c}, Dijana}, year = {2015}, pages = {46-50}, DOI = {10.1016/j.jviromet.2015.04.020}, keywords = {cloning, capsid protein, HEV, quasispecies, SSCP}, journal = {Journal of virological methods}, doi = {10.1016/j.jviromet.2015.04.020}, volume = {221}, issn = {0166-0934}, title = {The application of single strand conformation polymorphism (SSCP) analysis in determining Hepatitis E virus intra-host diversity}, keyword = {cloning, capsid protein, HEV, quasispecies, SSCP} }
@article{article, author = {\v{C}erni, Silvija and Prpi\'{c}, Jelena and Jemer\v{s}i\'{c}, Lorena and \v{S}kori\'{c}, Dijana}, year = {2015}, pages = {46-50}, DOI = {10.1016/j.jviromet.2015.04.020}, keywords = {cloning, capsid protein, HEV, quasispecies, SSCP}, journal = {Journal of virological methods}, doi = {10.1016/j.jviromet.2015.04.020}, volume = {221}, issn = {0166-0934}, title = {The application of single strand conformation polymorphism (SSCP) analysis in determining Hepatitis E virus intra-host diversity}, keyword = {cloning, capsid protein, HEV, quasispecies, SSCP} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE

Uključenost u ostale bibliografske baze podataka::


  • MEDLINE
  • Abstracts on Hygiene and Communicable Diseases
  • Elsevier BIOBASE
  • Pascal et Francis (INST-CNRS)
  • Reference Update
  • Tropical Diseases Bulletin
  • Scopus

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