Naslov
Phosphate release and uptake by Acinetobacter calcoaceticus in high strength phosphorus bearing wastewater

Autori
Hrenović, Jasna

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
Power of Microbes in Industry and Environment / Mrša V., Hajsig D. - Zagreb : Prehrambeno-biotehnološki fakultet Sveučilišta u Zagrebu, 2002

Skup
Power of Microbes in Industry and Environment

Mjesto i datum
Opatija, Hrvatska, 07.06.2000. - 09.06.2000

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Acinetobacter calcoaceticus; phosphorus; wastewater; phosphorus removal

Sažetak
In order to understand biological phosphorus removal mechanism, the role of various carbon sources and growth conditions applied at high initial concentrations of phosphorus (around 100 mg P L-1), on the phosphate release and uptake in pure culture of Acinetobacter calcoaceticus DSM1532 was investigated. P-release and uptake studies with various carbon sources were carried out in alternating anaerobic/aerobic batch experiments. The amount of P released in anaerobic stage and P accumulated in aerobic stage varied depending on the carbon source. The highest P-release resulted in the highest P-uptake rates. The highest P-release and P-uptake rates were obtained with sodium propionate as the carbon source (3.24 x 10-6 and 2.27 x 10-6 mg PO4-P cell-1), followed by sodium acetate (1.60 x 10-6 and 5.92 x 10-7 mg PO4-P cell-1). Glucose addition in the media containing propionate and acetate caused lower P-release and uptake ratios (6.83 x 10-8 and 2.60 x 10-8 mg PO4-P cell-1), but it did not have significant influence on the final percentage of P-removal and cell multiplication. Cultivation on the glucose as the sole carbon source resulted in P release during anaerobic and aerobic stage, without cell multiplication. Glucose feeding leaded to deterioration of P removal potential of A. calcoaceticus. Cultivation of A. calcoaceticus in sodium acetate in batch test without preliminary resort to the anaerobic conditions resulted in aerobic P uptake and cell multiplication. P-uptake rate (1.25 x 10-8 mg PO4-P cell-1) and percentage of P-removal were lower than those observed in anaerobic/aerobic test. A. calcoaceticus behaved as P-accumulating bacterium without the necessity of resorting to intermediate anaerobiosis. The semi-continuous test with sodium acetate was carried out to examine the adaptation possibility of A. calcoaceticus to the high phosphorus loads in the wastewater. The maximal P-uptake ratio (1.45 x 10-8 mg PO4-P cell-1) was achieved after 24 h of experiment. After 72 h of experiment P-removal, number of A. calcoaceticus and pH-value became almost stabile. The P-uptake ratios during that time varied between 1.25 x 10-10 and 8.28 x 10-11 mg PO4-P cell-1. Although the system showed capable to tolerate and reduce high levels of P in the synthetic wastewater, observed efficiency was poor in comparison with anaerobic/aerobic system. Nevertheless, observed P-removals were much higher than that observed in aerobic batch experiment; thus indicate the possibility of adaptation of A. calcoaceticus on the high phosphorus loads in the wastewater. The observed experimental results indicate the ability of phosphate-accumulating bacteria A. calcoaceticus DSM1532 to consume orthophosphate and accumulate it as polyphosphate in alternated anaerobic/aerobic conditions as well as in aerobic conditions without the necessity of preliminary resort to the anaerobic stage.

Izvorni jezik
Engleski

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