Pregled bibliografske jedinice broj: 756384
Alternating current voltammetric determination of DNA concentrations at a microgram per liter level
Alternating current voltammetric determination of DNA concentrations at a microgram per liter level // Analytical biochemistry, 156 (1986), 2; 454-462 doi:10.1016/0003-2697(86)90279-4 (međunarodna recenzija, članak, znanstveni)
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Naslov
Alternating current voltammetric determination of DNA concentrations at a microgram per liter level
Autori
Krznarić, Damir ; Ćosović, Božena
Izvornik
Analytical biochemistry (0003-2697) 156
(1986), 2;
454-462
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
DNA ; alternating current voltammetry ; mercury electrode
Sažetak
Alternating current voltammetry is used as a fast and highly sensitive method of DNA detection at a microgram per liter level. The method is based on the measurement of adsorption effects of denatured DNA at the hanging mercury drop electrode. The proposed procedure consists of thermal denaturation of DNA, which is followed by electrochemical detection of denatured DNA. A sharp adsorption peak of denatured DNA, at the potential of -1.4 V, is measured in 0.3 mol/liter NaCl and 0.03 mol/liter NaHCO3 (pH about 9) after an accumulation of DNA at the electrode surface. To enhance the sensitivity, the solution is stirred during adsorption. The influence of proteins, a polysaccharide, and RNA on the DNA determination was also studied.
Izvorni jezik
Engleski
Znanstvena područja
Kemija, Biologija
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE
